主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (5): 383-389.doi: 10.3969/j.issn.1006-2157.2016.05.007

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Antioxidant activities and chemical compositions of Thymus quinquecostatus Celak*

YAN Cheng1, CHEN Xiaoyi1, SUI Hong2, TIAN Yuxin1, WANG Yan1, BAI Shaojuan1, ZHAO Yicheng1, SHI Renbing1, SHE Gaimei 1#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Pharmacy College, Ningxia Medical University
  • Received:2016-02-19 Online:2016-05-30 Published:2016-05-30

Abstract: Objective To study the antioxidant activities and chemical compositions of Thymus quinquecostatus Celak (dijiao). Methods The antioxidant activities of five polar fractions of dijiao were screened and evaluated using DPPH and ABTS free radical scavenging assays. After the chemical compositions were separated and purified by using silica gel chromatography, Sephadex LH-20 chromatography and MCI GEL CHP-20P column chromatography, the chemical compositions were identified by MS and NMR spectrometers, and records of literature. Results From the EtOAc and n-BuOH fractions which showed high antioxidant activity, total 18 compositions were separated and identified: scutellarin (“1 ”short for peak No. 1, the same below), scutellarein (2), 5,6,7-trihydroxy-4′-methoxyflavone (3), xanthomicrol (4), apigenin (5), 4′-methoxyluteolin (6), luteolin-7-O-β-D-glucopyranoside (7), luteolin (8),and rutin (9), danshensu (10), vanillic acid (11), protocatechuic acid (12), chlorogenic acid (13), caffeic acid (14), ferulic acid (15), 2,6-dihydroxy-4-isopropylphenyl-β-D-glucopyranoside (16), olenolic cid (17) and daucosterol (18). Conclusion The composition 1,2, 3 and 16 were the first reported in the genus Thymus, and 4~8, 10~12, 14~15 and 17~18 were found first in dijiao.

Key words: Thymus quinquecostatus Celak, antioxidant activity, scutellarin, scutellarein, xanthomicrol

CLC Number: 

  • R284.1