主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (5): 399-405.doi: 10.3969/j.issn.1006-2157.2016.05.01

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Effects of electro-acupuncture on activity of microglia in spinal dorsal horn in rat model of transplantation of autologous nucleus pulposus*

JI Lili, GE Dongyu, REN Xiujun#, ZHAO Liyun, LI Genmao, TU Ya   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2015-11-06 Online:2016-05-30 Published:2016-05-30

Abstract: Objective To study the different prescriptions of eletcro-acupuncture (distal-proximal acupoints, proximal acupoints and distal acupoints) on pain-related behaviors and expression of microglia marker OX42 and cyclooxygenase 2 (COX2) in spinal dorsal horn in rat model of transplantation of autologous nucleus pulposus, so as to provide experimental basis for the mechanism of the different prescriptions. Methods 54 healthy male SD rats were randomly divided into six groups (each n=9)-normal group, model group, sham operation group, distal-proximal acupoint group, proximal acupoint group, distal acupoint group. After modeling, the rats in three acupuncture groups were treated for 20 minutes, once daily for consecutive 7 days. Those in distal-proximal acupoint group were needled at bilateral “L5 Jiajixue” (Ex-B2), “Dachangshu” (BL 25), “Weizhong” (BL 40) and “Kunlun” (BL 60); those in proximal acupoint group were needled at bilateral “L5 Jiajixue” (Ex-B2)and “Dachangshu” (BL 25) ; the rats in distal acupoint group rats were needled at bilateral “Weizhong” (BL 40)and “Kunlun” (BL 60). Thermal withdrawal latencies were evaluated. The expressions of OX42 and COX2 in the left spinal dorsal horn were detected using immunohistochemical methods. Results Thermal withdrawal latencies and the expression of OX42 and COX2 increased in rat model of transplantation of autologous nucleus pulposus. After treatment, thermal pain threshold was improved (P<0.05), and the expression of OX42 and COX2 were inhibited (P<0.05). The inhibitory effects of distal-proximal acupoint group and proximal acupoint group were superior to that in distal acupoint group. Conclusion Each acupuncture prescription reduced anti-inflammatory effects by inhibiting inflammatory signaling pathways in spinal dorsal horn, with the prescription of distal-proximal acupoints and proximal acupoints superior to distal acupoints.

Key words: electro-acupuncture, low back pain, inflammatory pain, nucleus pulposus, spinal dorsal horn, microglia, cyclooxygenase2 (COX2), rats

CLC Number: 

  • R245.31+9