主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (5): 406-412.doi: 10.3969/j.issn.1006-2157.2016.05.011

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Effects of moxibustion at Zusanli(ST36)on endogenous protective factors and related proteins expression in gastric mucosal lesions rats after damaging nucleus tractus solitarii*

XIANG Juan, CHEN Guo, OUYANG Lizhi, LI Fei, XIANG Liting, CHEN Ying, YU Jun, YANG Zhou, LI Tielang, PENG Liang#   

  1. School of Acupuncture and Moxibustion and Tuina, Hunan University of Chinese Medicine, Hunan 410208
  • Received:2015-11-24 Online:2016-05-30 Published:2016-05-30

Abstract: Objective To study the role of nucleus tractus solitarii (NTS) on the protective effects of moxibustion on gastric mucosa lesion in the nervous pathway by investigating the effects of moxibustion at "Zusanli" (ST 36) on endogenous protective factors and gap-associated protein expression of gastric mucosal lesion rats after damaging their nucleus tractus solitarii (NTS). Methods 48 healthy SD rats, SPF grade, were divided into four groups using random number table(each n=12): normal group, model group, moxibustion group, moxibustion + NTS damaged group. Three days after bilateral NTS damaged in moxibustion + NTS damaged group, gastric mucosal lesion rat model was induced by intragastrical (i.g.) administration of anhydrous ethanol at dose of 6 mL/kg and aspirin mixed suspension at dose of 200 mg/kg for consecutive three days. The next day, rats in moxibustion group and moxibustion + NTS damaged group were treated by moxibustion at Zusanli (ST 36) twice daily for consecutive three days. All rats were sacrificed, then their serum and gastric tissue were collected. Ulcer index (UI) was calculated, and the expressions of epidermal growth factor (EGF), nitric oxide (NO), Bcl-2 anti-apoptotic proteins (Bcl-2) and pro-apoptotic protein (Bax) were detected. Results Compared with normal group, UI in model group increased significantly(P<0.05), decreased in moxibustion group(P<0.05). Compared with model group, content of EGF and NO in serum and gastric tissue increased remarkably in moxibustion group(P<0.05)which were higher than those in moxibustion + NTS damaged group(P<0.05). Protein expression of Bcl-2 increased significantly in moxibustion group and moxibustion + NTS damage group compared with model group, while expression of Bax protein decreased with increasing of AI (P<0.05). Conclusion Treatment of moxibustion at Zusanli (ST 36) can regulate the expression of endogenous protective factors and proteins to protect rats’ gastric mucosa, which was affected by damaging NTS. It is suggested that NTS be the important regulatory location in the signaling pathway of protective effects of moxibustion at Zusanli on gastric mucosal.

Key words: moxibustion, damage nucleus tractus solitarii, gastric mucosal lesions, rats

CLC Number: 

  • R245.81