主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (7): 555-561.doi: 10.3969/j.issn.1006-2157.2016.07.007

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Regulatory effect of Zhuanggu Zhitong Capsule on Treg/Th17 balance in rats with osteoporosis induced by ovariectomy*

ZENG Ying1, LI Weijuan2, ZHANG Wenjuan2, LI Jinping2#, LEI Xiaoming3, MO Xinmin3   

  1. 1 The First Affiliated Hospital, Hunan University of Traditional Chinese Medicine, Hunan410007;
    2 School of Pharmacy Science, Central South University;
    3 Hunan University of Traditional Chinese Medicine
  • Received:2016-01-11 Online:2016-07-30 Published:2016-07-30

Abstract: Objective To investigate the anti-postmenopausal osteoporosis mechanism of Zhuanggu Zhitong Capsule (Bone-strengthening Pain-relieving Capsule, ZGZTC) in regulating Treg/Th17 balance in bone tissue.Methods Seventy-two 8-month-old SD female rats were allocated randomly into sham operation group, model group, estradiol group (0.21 mg/kg), ZGZTC high-dose group (1.28 g/kg), ZGZTC medium-dose group (0.64 g/kg) and ZGZTC low-dose group (0.32 g/kg). There were 12 rats in each group. Bilateral ovariectomy was performed on rats in all groups except for the sham operation group. After administration for 18 weeks, all rats were anaesthetized intraperitoneally with hydrazine hydrate. The content of E2, IL-10 and IL-17A was measured with ELISA in left femur. Gene expression of Foxp3, RORγt, IL-17A and IL-10 in right femur was detected by using RT-PCR, and the number of cell with RORγt or Foxp3 was counted with immunohistochemistry in left tibia. Results Zhuanggu Zhitong Capsule significantly increased E2 and IL-10 levels in bone tissue (P<0.01), reduced the level of IL-17A in bone tissue (P<0.05 or P<0.01). It significantly increased the Foxp3 and IL-10 mRNA expression and inhibited the RORγt mRNA expression in bone tissue (P<0.05). The percentage of cell with Foxp3 was significantly increased by ZGZTC in bone tissue (P<0.05), and the percentage of cell with RORγt was significantly reduced (P<0.05 or P<0.01). The ratio of Foxp3 to RORγt presented a positive correlation with the E2 level (r=0.990 5,P<0.01). Conclusion Zhuanggu Zhitong Capsule could downregulate the number and function of Th17, upregulate the number and function of Treg in bone tissue in ovariectomized rats partly via improving the E2 level.

Key words: Zhuanggu Zhitong (Bone-strengthening Pain-relieving) Capsule, Treg/Th17, postmenopausal osteoporosis, rats

CLC Number: 

  • R285.5