主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (7): 568-574.doi: 10.3969/j.issn.1006-2157.2016.07.009

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Effects of Herba Ephedra and Fructus Schisandrae combined with dexamethasone on angiogenic factors and inhibitors in rats with idiopathic pulmonary fibrosis*

ZHANG Xiaojuan, ZHANG Tian, TIAN Weilan, LI Rui, ZHAI Huaqiang#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2016-01-19 Online:2016-07-30 Published:2016-07-30

Abstract: Objective To observe the effects of Mahuang(Herba Ephedra) and Wuweizi(Fructus Schisandrae) combined with dexamethasone(DXM)on expression of angiogenic factors and inhibitors in rats with idiopathic pulmonary fibrosis (IPF).Methods Wistar male rats of SPF level (n=106) were divided randomly into sham operation group, model group, Mahuang group, Wuweizi group, Mahuang+DXM group, Wuweizi+DXM group and DXM group. The model of IPF was established by using instillation of bleomycin (7 mg/ kg) except for the sham operation group. Each group was administered intragastrically of corresponding drug. All rats were sacrificed on the 7th and 28th day via abdominal aorta blood-letting, and the pathological changes of lung tissue were evaluated with HE staining and the expression of hypoxia-inducible factors 1α(HIF-1α), platelet derived growth factor(PDGF), pigment epithelium-derived factor(PEDF) and endostatin in rat serum were also measured with ELISA. Results Compared with the sham operation group, the expression of HIF-1α,PDGF, PEDF and endostatin in model group significantly increased (P<0.01); the HIF-1α content on the 28th day was higher than that on the 7th day, and the PEDF and endostatin content were lower than that on the 7th day. The expression of HIF-1α and PDGF significantly decreased in Mahuang group and Wuweizi group compared with the model group, and the expression of PEDF and endostatin increased(P<0.05). Compared with Mahuang group on the 28th day,the expression of HIF-1α and PDGF on the 7th day increased markedly, but the expression of PEDF and endostatin on the 28th day was lower than those on the 7th day. In the Wuweizi group the changes in the expression of HIF-1α,PDGF, PEDF and endostatin were opposite to Mahuang group. The expression of HIF-1α and PDGF significantly decreased in Mahuang+DXM group and Wuweizi+DXM group compared with the model group(P<0.05), and the expression of PEDF and endostatin increased. In Wuweizi+DXM group the HIF-1α content was significantly higher than those in DXM group while the PEDF content was significantly lower(P<0.05). Conclusion Regulatory factors on angiogenesis lose their balance in rats with IPF. Mahuang and Wuweizi could achieve inhibition of the expression of HIF-1α and PDGF, and selectively promote the expression of PEDF and endostatin. When combined with DXM, the selectivity might be weakened in different pathological stages.

Key words: Mahuang(Herba Ephedra), Wuweizi(Fructus Schisandrae), dexamethasone, idiopathic pulmonary fibrosis, angiogenesis, rats

CLC Number: 

  • R285.