主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (7): 580-585.doi: 10.3969/j.issn.1006-2157.2016.07.011

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Effect and mechanism of oldenlandia on Cxcl1 gene expression in macrophages*

YUE Xiaoning, SHI Yang, ZANG Shizhu#   

  1. Biotechnology Department, Dalian Medical University, Liaoning 116044
  • Received:2016-02-23 Online:2016-07-30 Published:2016-07-30

Abstract: Objective To explore the immunoregulatory effect of Oldenlandia (Hedyotis diffusa Villd.), by investigating on the expression of macrophage cytokines and on TLR-4 inflammatory signal transduction pathway, aiming to propose a new experimental and theoretical evidence for the use of oldenlandia.Methods Three common cytokines, such as IL-6, MCP-1 and Cxcl1 in macrophage cells were detected by using RT-PCR after treatment with oldenlandia solution. Autodock software was used to analyze the correlation between the main components and TLR-4 so as to explore the mechanism of expression changes of the cytokines. Results Oldenlandia solution could upregulate the expression of Cxcl1, which might depend on the combination of TLR4 and stigmasterol-beta-D-glucoside, trans-4-methoxycinnamyl alcohol, and asperulosidic acid. Conclusion Oldenlandia could activate TLR-4 signal pathway and thus affect the expression of Cxcl1 and function of macrophage. It can be inferred that oldenlandia could regulate immunity, which could serve as the theoretical rationale for the development of new drugs in the laboratory.

Key words: Baihuashe Shecao (Hedyotis diffusa Villd.), macrophage, Cxcl1 gene, Toll like receptor 4

CLC Number: 

  • R285.5