主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (8): 670-678.doi: 10.3969/j.issn.1006-2157.2016.08.012

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Three-step sequential therapy on pathomorphology of bronchial lung tissues of asthmatic rat airway remodeling after hormone intervention*

QIN Fangfang1, CHENG Juan2, CUI Hongsheng3#, TIAN Yan3, LI Chang’an3, QIN Yang3, LI Qiannan3, SHENG Ruihong4, PAN Zi’ang4, LIU Baoshan1   

  1. 1 Department of Chinese Medicine, Xinhua Hospital of Beijing Tongzhou District, Beijing 101100;
    2 Department of Pathology , Dongzhimen Hospital, Beijing University of Chinese Medicine;
    3 Respiratory Department, Third Affiliated Hospital of Beijing University of Chinese Medicine;
    4 Department of Electron Microscopic Laboratory, China-Japan Friendship Hospital
  • Received:2016-03-16 Online:2016-08-31 Published:2016-08-31

Abstract: Objective To observe the effect of three-step sequential therapy on the pathomorphology of the bronchial lung tissues of rat asthmatic model with airway remodeling after hormone intervention using light microscope and electron microscope. Methods The airway remodeling of asthmatic rats model was induced by hormone intervention to simulate hormone withdrawal (HW) of steroid dependent asthma (SDA) patients. Altogether 150 rats randomly divided into five groups (each n=30) of airway remodeling group, dexamethasone intervention group, pulmicort group, three-step sequential therapy group and normal control group. Morphological changes of the bronchial lung tissues of rats of all groups were observed under light microscope and electron microscope, and thicknesses of the bronchial walls and the bronchial smooth muscle and the number of the cell nucleus in the bronchial smooth muscle were measured. Results Using HE staining, the lung tissues of rats except for those in normal control group were infiltrated by inflammation cells with different degrees, but the rats of three-step sequential therapy group were observed significantly slight inflammatory reaction. Using Masson trichromatic staining, massive collagen fiber in pulmonary interstitial of airway remodeling group deposited, while deposition increased during hormone withdrawal in dexamethasone intervention group, and significantly less deposition was observed in both three-step sequential therapy group and pulmicort group. Before HW, the thickness of the bronchial walls in three-step sequential therapy group decreased significantly compared with that in airway remodeling group(P<0.01); during and after HW, the thickness of the bronchial walls in three-step sequential therapy group decreased compared with dexamethasone intervention group and airway remodeling group(P<0.01,P<0.05). Before HW, there is no difference (P>0.05) of bronchial smooth muscle thickness observed in all the medication groups; during and after HW, bronchial smooth muscle thickness decreased compared with dexamethasone intervention group and airway remodeling group(P<0.01,P<0.05). The number of cell nucleus in the bronchial smooth muscle of three-step sequential therapy group decreased significantly, regardless of HW, compared with dexamethasone intervention group and airway remodeling group(P<0.01) . Using electron microscope, airway epithelial cilia falling off was remarkably alleviated during HW in three-step sequential therapy group and pulmicort group, while that in dexamethasone intervention group during or after HW was the same as the airway remodeling group. Conclusion Three-step sequential therapy could inhibit the airway remodeling by the way of relieving inflammatory changes of the bronchial lung pathomorphology of rat asthmatic model.

Key words: asthmatic rat airway remodeling after hormone intervention, three-step sequential therapy, pathomorphology of the bronchial lung tissues, rats

CLC Number: 

  • R285.5