主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (8): 679-984.doi: 10.3969/j.issn.1006-2157.2016.08.013

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Hydroxysafflor yellow A inhibited abnormal proliferation of vascular endothelial cells*

WANG Xixi1,WANG Jingjing1,WANG Xu1,FAN Angran1,YU Xue1, HU Jinghong1,XIE Hua1,XU Yingying1,LIU Li1,ZANG Baoxia2,ZHANG Qian1#   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine,Beijing 100029;
    2 Department of Pharmacology, Beijing Cardiovascular and Pulmonary Diseases Institute
  • Received:2016-03-20 Online:2016-08-31 Published:2016-08-31

Abstract: Objective To study the inhibitory mechanism of hydroxysafflor yellow A (HSYA) on abnormal proliferation of vascular endothelial cells. Methods Co-cultured model in vitro was established, with supernatant fraction of LS180 human colon adenocarcinoma cells tumor cells and ECV304 human umbilical vein endothelial cells. Then mRNA expressions of vascular endothelial growth factors (VEGF) and VEGF receptors (KDR), basic fibroblast growth factor (bFGF)and bFGF receptors(bFGFR), proteoglycan related to cell proliferation (HSPG2), p53 and c-myc were detected using real-time fluorescence quantitative PCR; protein expressions of VEFG, KDR, bFGF and bFGFR in supernatant fraction were measured by ELISA. Results In the cell co-cultured model, the HSYA concentration of 0.66 and 0.33 mg/L inhibited mRNA and protein expressions of VEGF,KDR, bFGF and bFGFR ,inhibited mRNA expressions of c-myc and HSPG2, while upregulated p53 mRNA expression. Conclusion HSYA inhibited angiogenesis and abnormal proliferation of vascular endothelial cells in co-cultured model by regulating expressions of VEGF,VEGFR, bFGF, bFGFR, HSPG2, c-myc, wild type p53. HSYA may be a potential tumor angiogenesis inhibitor.

Key words: hydroxysafflor yellow A, vascular endothelial cell, vascular endothelial growth factor (VEGF), tumor, LS180 human colorectal adenocarcinoma cells

CLC Number: 

  • R285.5