主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (10): 833-838.doi: 10.3969/j.issn.1006-2157.2016.10.010

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Effects of Chinese medicine compound of warming and moistening lung with tonifying spleen on guinea pigs with allergic rhinitis*

XU Yan1,KONG Deming1,CHU Huilun1,YANG Ying1,YAN Yue2,LI Youlin 2 ,SUN Wenyan1#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102;
    2 Key Institute of State Administration of Traditional Chinese Medicine of Pneumonupathy Chronic Cough and Dyspnea, China-Japan Friendship Hospital
  • Received:2016-05-10 Online:2016-10-30 Published:2016-10-30

Abstract: Objective To study the effects of Chinese medicine compound of warming and moistening lung with tonifying spleen(hereinafter“TCM”) on behavior, serum IgE and IL-6, as well as nasal mucosa pathological changes of guinea pigs with allergic rhinitis. Methods SPF grade guinea pigs, half male and half female, were randomly divided into normal group, model group, Biyankang group (0.41 g/kg), loratadine group (0.91 mg/kg), TCM high-dose group (3.04 g/kg), mid-dose group(1.52 g/kg) and low-dose group(0.76 g/kg). Except the normal group, other groups were followed by bilateralnostrils intranasal inhalation once daily with 10% TDI olive oil solution(5 μL each nostril), 7 days in a row. After the model was established successfully, the guinea pigs were i.g. corresponding medication, with dripping TDI continuously for 14 days every other day. Nasal symptoms of all groups were observed and recorded. The serum contents of IgE and IL-6 were determined by ELISA, nasal mucosa pathological morphological changes were observed under light microscope, and number of eosinophils (EOS) were counted. Results Compared with the model group, scores of behavioral symptom were significantly lower in both TCM high- and mid- dose group (P<0.01 or P<0.05), and serum content of IgE and IL-6 significantly lower in all the TCM groups (P<0.01 or P<0.05). High-dose and mid-dose of TCM relieved EOS infiltration of nasal mucosa,compared with model group (P<0.01 or P<0.05), furthermore, in TCM high dose group, no exposure-related nasal lesions or no flammatory cell infiltration was found (P<0.05). Conclusion TCM could improve the behavior of guinea pigs with allergic rhinitis and reduce serum IgE and IL-6 levels, relieve the nasal mucosa pathological changes.

Key words: Chinese medicine compound of warming and moistening lung tonifying spleen, allergic rhinitis, behavior, pathology, IgE, IL-6, guinea pigs

CLC Number: 

  • R285.5