主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (11): 893-901.doi: 10.3969/j.issn.1006-2157.2016.11.003

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Study of liver fibrosis rat model with pattern of liver-qi stagnation and validation of pattern time window by TCM formulas treatment

YAN Jinglu, XUE Xiaoxing, LI Junling,XIA Kai, WANG Tian, WANG Wei#   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2016-06-30 Online:2016-11-30 Published:2016-11-30

Abstract: Objective To explore the pathogenesis and TCM patterns related to liver fibrosis , as well as to establish the rat model of liver fibrosis of combination of disease and pattern. Methods The rat liver fibrosis model with pattern of liver-qi stagnation was induced by subcutaneous injection of carbon tetrachloride. The patterns were distinguished by body weight, open field test, grip strength test, and form collection of symptoms and signs, and the pathological stage was confirmed by HE and Masson staining. Then Chaihu Shugan Powder, Xiaoyao Powder and Sini Powder were used to direct at TCM patterns, and colchicine was used to direct at the disease. Results Four weeks after modeling, pattern of liver-qi stagnation occured on the majority of liver fibrosis rats and lasted to the twelfth week. On the other hand, the main pathological stage was S2 to S3 at the fourth week after modeling, S3 at the eighth week and S4 at the twelfth week. The treatment of TCM formulas validated the improvement of symptoms and pathological stages. Conclusion The time window of TCM pattern of liver fibrosis rat model with pattern of liver-qi stagnation was from 4 to 10 weeks, which was proved by TCM formulas. And the model of combination of pattern and disease was reliable.

Key words: liver fibrosis, pattern of liver-qi stagnation, rat model, time window of pattern

CLC Number: 

  • R285.5