主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (11): 902-908.doi: 10.3969/j.issn.1006-2157.2016.11.004

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Effect of Jianpi Huashi Tongluo Formula on synovial angiogenesis in rats with adjuvant arthritis

ZHANG Xiaojun1, LIU Jian2# ,WAN Lei2 ,HUANG Chuanbing2   

  1. 1 Clinical School of Chinese Medicine, Anhui University of Chinese Medicine,Anhui 230038;
    2 First Affiliated Hospital, Anhui University of Chinese Medicine
  • Received:2016-06-29 Online:2016-11-30 Published:2016-11-30

Abstract: Objective To observe the mechanism of Jianpi (invigorating the spleen) Huashi (resovling dampness) Tongluo (removing obstruction in collaterals) Formula (JHTF) on PI3K/AKT/mTOR signaling pathway, hypoxia inducible factor (HIF-1α), vascular endothelial growth factor (VEGF-A) ,microvessel density (MVD) and endostatin (ES) of synovial tissue in rats with adjuvant arthritis (AA). Methods Fifty rats were randomly divided into five groups — normal control group, model group, methotrexate group, tripterygium glycosides tablet group and JHTF group. Rat AA model was induced by using Freund's complete adjuvant. After modeling, each treatment group were i.g. corresponding medication for consecutive 30 days, and then the expression of microvascular density (MVD) was detected using immunohistochemical, interleukin (IL)-6, IL-10, HIF-1α and VEGF were measured by enzyme-linked immunosorbent assay, and PI3K, AKT1, p-AKT1, mTOR and ES proteins were detected by Western blotting. Results Compared with normal control group, footpad swelling, arthritic index increased, serum IL-6, VEGF and HIF-1α and MVD, PI3K, AKT1, p-AKT1, mTOR, ES in synovial vascular significantly increased in the model group(P<0.05 or P<0.01). Compared with the model group, the MVD count, serum VEGF-A, HIF-1α and IL-6 decreased in JHTF group. The expressions of PI3K, p-AKT1, mTOR, ES in synovial vascular in JHTF group were lower ,and serum IL-10 was higher than those of the model control group. Conclusion Jianpi Huashi Tongluo Formula can improve angiogenesis by regulating PI3K/AKT/mTOR pathway, expression of HIF-1α and ES in rat AA model.

Key words: Jianpi Huashi Tongluo Formula, adjuvant arthritis, angiogenesis, PI3K/AKT/mTOR pathway, rats

CLC Number: 

  • R285.5