主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (11): 909-914.doi: 10.3969/j.issn.1006-2157.2016.11.005

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Effect of Jianpi Yiqi Huayu Jiedu Formula on proliferation, cycle and apoptosis of HCT116 colorectal cancer cells

JIAN Xiaolan1, YANG Xiao1, LUO Ji2, LI Yongmin2, LYU Yuan2, TAN Xiaoning2, JIANG Yilan2#(1 Graduate School of Hunan University of Chinese Medicine, Hunan 410208; 2 The Affiliated Hospital of Hunan Academy of Chinese Medicine)   Abstract: Objective To study the effect of medicated serum of Jianpi Yiqi Huayu Jiedu Formula   

  1. 1 Graduate School of Hunan University of Chinese Medicine, Hunan 410208;
    2 The Affiliated Hospital of Hunan Academy of Chinese Medicine
  • Received:2016-06-03 Online:2016-11-30 Published:2016-11-30

Abstract: Objective To study the effect of medicated serum of Jianpi Yiqi Huayu Jiedu Formula (JYHJF), a TCM compound formulated according to TCM principle of tonifying spleen, replenishing qi, removing blood stasis and detoxification, on proliferation, cycles, apoptosis of HCT116 colorectal cancer cells and on the protein expression of Caspase-3,Bcl-xL and Bax of HCT116 cells. Methods After drug-contained serum (10%, 15%, 20%) treated HCT116 cells for 12, 24, 48 and 72 h, the proliferation was detected by MTT method; after treated for 24 or 48 h, the cell cycle and apoptosis rate were detected by propidium iodide (PI) staining. The protein expression level of Caspase 3, Bcl-xL and Bax in HCT116 cells were detected by Western blot. The control group was treated by blank serum and 10% fetal bovine serum (FBS). Results Compared with the same concentration of blank serum group, the cell proliferation was inhibited significantly in 15% and 20% medicated serum group after 12 h (P<0.05), and the inhibitory effect showed time- and concentration- dependence with the highest at 72 h (P<0.05). In 10% and 15% medicated serum group, cells in G2 phase decreased, but the G1-phase cells and S-phase cells increased; cells in G1 phase and S phase increased and those in G2 phase decreased in 10% medicated serum group with the remarkable Results at 48 h. It was suggested that the medicated serum could blocked cell cycles, with the positive correlation with time. Compared with the blank serum groups at the same concentration, after treated 24 and 48 h, apoptosis rate of HCT116 cells increased, and had the positive correlation with time and concentration. Compared with the blank serum group, the expression of Bax protein was upregulated and that of Caspase-3 and Bcl-xL were downregulated in 15% medicated serum group (P<0.05). Conclusion The effect of Jianpi Yiqi Huayu Jiedu Formula on inhibiting proliferation and inducing apoptosis may be via the way of blocking HCT116 cells cycles and of upregulating Bax protein expression with downregulating Caspase-3 protein and Bcl-xL protein expression.

Key words: Jianpi Yiqi Huayu Jiedu Formula , HCT116 colorectal cancer cells , proliferation, cell cycle, apoptosis

CLC Number: 

  • R285.5