主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (11): 933-940.doi: 10.3969/j.issn.1006-2157.2016.11.009

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Absorbed constituents in rat serum after oral administration of water extract of Simiao Yong’an Decoction

CHI Sensen1, WANG Weihua2, LIU Bin1#   

  1. 1 School of Chinese Medica Materia, Beijing University of Chinese Medicine, Beijing 100102;
    2 National Institute of Metrology, China
  • Received:2016-04-20 Online:2016-11-30 Published:2016-11-30

Abstract: Objective To study the absorbed constituents of water extract of Simiao Yong’an Decoction (SYD) in serum of rats, so as to find out the pharmacodynamics material basis. Methods Using liquid chromatograph-mass spectrometry (LCMS)-ion trap (IT)-time of flight (TOF) method under both positive and negative ion modes of electrospray ionization, the water extract of SYD, rats’ serum after oral administration of SYD and the blank serum were analyzed. Serum samples were pretreated by using methanol. The chromatographic condition consisted of Inertsil ODS-3 column, mobile phase of acetonitrile (A) and water (0.1% formic acid contained) (B) with gradient elution. The compounds absorbed in serum was identified by multiple stage mass spectrum fragments, published reports and standard substances. Results Finally, 14 compounds were preliminary identified in rats serum, of which ten compounds were prototypes of SYD and four were metabolites. Nine peaks were validated as harpagide, chlorogenic acid, loganin, sweroside, liquiritin, hyperoside, angoroside C, isochlorogenic acid C and harpagoside. Conclusion Constituents of SYD in serum were generated from compatibility of all herbal medicines. This study would contribute to the further study of pharmacodynamics properties and metabolism of SYD.

Key words: Simiao Yong’an Decoction, constituents absorbed in serum, liquid chromatograph-mass spectrometry-ion trap-time of flight, metabolites

CLC Number: 

  • R284.1