主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (12): 989-997.doi: 10.3969/j.issn.1006-2157.2016.12.005

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Mechanism of tetramethylpyrazine in treatment of coronary heart disease based on the coexpression-protein interaction network*

HUO Mengqi,ZHANG Yanling,ZHENG Shichao,QIAO Yanjiang#   

  1. Research Center of Traditional Chinese Medicine Information Engineering,Beijing University of Chinese Medicine,Beijing 100102
  • Received:2016-07-22 Online:2016-12-30 Published:2016-12-30

Abstract: Objective To explore the mechanism of tetramethylpyrazine in the treatment of coronary heart disease (CHD) based on the coexpression-protein interaction network analysis. Methods The targets’ information of tetramethylpyrazine was obtained from pharmacophore-based virtual screening and database searching from STITCH & Chemprot, and the protein-protein interactions of the targets were retrieved by String database. The protein interaction network was constructed by Cytoscape. Next, the coexpression-protein interaction networks were constructed by integrating gene-expression profile under physiological and disease conditions. The modules of disease associated coexpression-protein interaction network were clustered and functional annotated based on fast agglomerate algorithm based on the edge clustering coefficients (FAG-EC) and gene ontology (GO) enrichment, by which the function modules were mapped to the coexpressin-protein interaction network under the normal physiological state to get the corresponding function modules. Then the function modules under two different conditions were compared and analyzed. Results The effect of tetramethylpyrazine on CHD was achieved by means of regulating and controlling immunological regulation or inflammatory response, cell apoptosis signaling pathway, blood circulation, heme metabolism and basal metabolism; myeloperoxidase (MPO) and hemeoxygenase-1 (HMOX1) probably were two key targets in treatment of CHD. Conclusion The mechanism and the key targets of tetramethylpyrazine in the treatment of CHD were illuminated at the molecular networks level, which could provide reference for clinical practice.

Key words: tetramethylpyrazine, coronary heart disease (CHD), function modules, mechanism

CLC Number: 

  • R9-39