主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (12): 998-1005.doi: 10.3969/j.issn.1006-2157.2016.12.006

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Hypolipidemic effect and mechanism of Semen Astragali Complanati on hyperlipidemia rats with kidney-yang deficiency pattern*

LIU Jing, WANG Jingxia#, GAO Fei, TANG Xiaoran, LI Siming, SUN Tianheng, ZHANG Chunguang, ZHANG Jianjun   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2016-07-22 Online:2016-12-30 Published:2016-12-30

Abstract: Objective To explore the effect of water extracts from Semen Astragali Complanati (Flatstem Milkvetch Seed, hereinafter“SAC”) on lipid metabolism in hyperlipidemia model rats with kidney-yang deficiency pattern, so as to try to discover the mechanism. Methods Female 10-week-old SD rats were randomly divided into six groups (each n=10): normal group, model group, estrogen group, SAC low-dose group, SAC mid-dose group and SAC high-dose group. The rat model was induced by bilateral ovariectomization combined with high-fat diet feeding. And rats of normal group and model group were intragastrically (i.g.) administered with saline, those of estrogen group were i.g. with estradiol valerate (0.2 mg/kg), and three SAC groups were i.g. with SAC extract at the dose of 34, 68 and 136 mg/kg for consecutive 8 weeks. The body weight of rats were recorded, serum TC, TG, HDL-C and LDL-C were measured and serum E2, FSH and estrogen receptors (ERα, ERβ) mRNA in liver tissue were detected. Results Compared with the normal group, the body weight of rats of model group increased significantly after three weeks’high-fat feeding (P<0.001); from Week 5 the level of total cholesterol(TC) and low density lipoprotein cholesterol(LDL-C) increased significantly (P<0.001 or P<0.01), and from Week 7 triglyceride(TG) and high density lipoprotein cholesterol(HDL-C) also did (P<0.001 or P<0.05); serum E2 significantly decreased (P<0.01), and follicle stimulating hormone(FSH) increased (P<0.05), the expression of ER-α mRNA in liver significantly decreased(P<0.05). High dose of SAC significantly reduced the serum level of TG and TC from Week 7 (P<0.05 or P<0.001), middle dose of SAC reduced the level of LDL-C and increased HDL-C significantly at the same time point (P<0.05, P<0.01), and a more week later, the low dose increased the serum level of HDL-C (P<0.05). Despite the levels of E2 and FSH of model rats showed no significant difference after treatment by SAC (P>0.05), the expression of ER-α mRNA in liver tissue increased in all SAC groups (P<0.05). Conclusion The effect of regulating blood lipid metabolism of SAC had been proved. The potential mechanism could be via upregulating the expression of estrogen receptors to amplify the signals transmitted by the receptors, which may make up the low estrogen level to direct at the disorder of lipid metabolism during peri-menopausal period.

Key words: Semen Astragali Complanati, estrogen, estrogen receptor, blood lipid metabolism, rats

CLC Number: 

  • R285.5