主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (12): 1017-1021.doi: 10.3969/j.issn.1006-2157.2016.12.009

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Effect of wind-dispelling drugs and deficiency-tonifying drugs on axonal growth inhibitory signaling pathway Nogo-A/NgR and RhoA/Rock2 after cerebral ischemia in rats:a developmental study on Hou’s Hei San*

LU Yue, ZHAO Hui, YAO Xiaoquan,CHANG Jiahui,ZHANG Qiuxia#   

  1. School of Traditional Chinese Medicine, Capital Medical University,Beijing 100069
  • Received:2016-07-20 Online:2016-12-30 Published:2016-12-30

Abstract: Objective To explore the effect of wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San, a TCM formula used in treatment of apoplexy, on axonal growth inhibitory factors Nogo-A,NgR, RhoA, Rock2 of rats with cerebral ischemia. Methods The model of permanent middle cerebral artery occlusion ( pMCAO) was induced by using Longa method. All rats were randomly divided into sham-operation group, model group, wind-dispelling drugs group(7.70 g/kg), deficiency-tonifying drugs group(2.59 g/kg) and combined drugs group(10.50 g/kg). After operation, each 12 h the rats were intragastrically administered for 72 h. Then the rats were sacrificed and their brain tissue were resected. The protein expression of Nogo-A, NgR, RhoA and Rock2 in hippocampus CA1 were detected by using Western blot. Results The protein expressions of Nogo-A, NgR, RhoA and Rock2 in model group increased significantly, compared with sham-operation group(P<0.01). Compared with model group, the expression of NgR in wind-dispelling drugs group, deficiency-tonifying drugs group and combined drugs group decreased remarkably(P<0.01). The expression of Nogo-A and Rock2 decreased as well, but only combined drugs group showed a statistically difference(P<0.05). Conclusion The effect of wind-dispelling drugs and deficiency-tonifying drugs of Hou’s Hei San against cerebral ischemia damage would be via regulating axonal growth inhibitory signal pathway Nogo-A/RhoA/ Rock2 to promote the recovery of neural function.

Key words: cerebral ischemia, wind-dispelling drugs, deficiency-tonifying drugs, Nogo-A/RhoA/Rock2 signaling pathway, rats

CLC Number: 

  • R285.5