主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2016, Vol. 39 ›› Issue (12): 1022-1026.doi: 10.3969/j.issn.1006-2157.2016.12.010

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Effect of Yijing Fang on expression of junctional adhesion molecules and zonula occludens in testicular sertoli cells of adenine-induced infertile rats*

ZHENG Xiaoting1, CHEN Dong2 , YIN Shen3, MA Ling1, WANG Jiahui2#   

  1. 1 Guiyang University of Chinese Medicine,Guizhou 550002;
    2 Hainan Medical University;
    3 The Chinese Medicine Hospital of Shenyang
  • Received:2016-04-11 Online:2016-12-30 Published:2016-12-30

Abstract: Objective To observe the effect of Yijing Fang, a TCM compound, on the expression of junctional adhesion molecules (JSM)and zonula occludens (ZO) in testicular sertoli cells of infertile rats with kidney-yang deficiency pattern induced by adenine. Methods Altogether 48 healthy male Wistar rats of SPF grade, two-month-old, were divided according to random number table, into blank group, model group, Fufang Xuanju group and Yijing Fang group(n=12 each). After modeling, the rats were intragastrically administered by corresponding medicines for consecutive 20 days. Then the rats were sacrificed and their testis and epididymis were resected. The sperm density and sperm activity were calcula-ted using Computer Assisted Sperm Analysis System, and the expression of JAM and ZO in testicular sertoli cells were measured using immunohistochemical method. Results Compared with the normal group, the sperm density, sperm activity rate and expression of JAM and ZO declined significantly(P<0.01). Compared with the model group, the sperm density and the sperm activity rate increased in Fufang XuanJu group and Yijing Fang group remarkably (P<0.05), while expression of JAM and ZO increased in Yijing Fang group((P<0.05). Conclusion Yijing Fang can repair the damage of testicular sertoli cells of kidney-yang deficiency pattern rats induce by adenine, via regulating the expression of JAM and ZO.

Key words: Yijing Fang, adenine, infertile, pattern of kidney-yang deficiency, junctional adhesion molecules, zonula occludens, rats

CLC Number: 

  • R285.5