主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (1): 20-26.doi: 10.3969/j.issn.1006-2157.2017.01.005

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Effect of hedysarum polybotrys polysacchcaide on TGF-β1/Smads signaling pathway in db/db mice with diabetic cardiomyopathy*

JIN Zhisheng1, WANG Dongxu1,ZHANG Huazhi1,HE Cailing1,NAN Xiangping1,WANG Qian2,CHU Huiyuan1   

  1. 1 Gansu University of Traditional Chinese Medicine,Gansu 730000;
    2 First Hospital of Chinese Medicine of Baoding,Hebei
  • Received:2016-09-02 Online:2017-01-30 Published:2017-01-30

Abstract: Objective To explore the effect of hedysarum polybotrys polysacchcaide (HPS) on myocardial fibrosis of db/db mice with diabetic cardiomyopathy (DCM) by taking TGF-β1/Smads signaling pathway as the index. Methods Altogether 60 six-week-old male db/db mice were randomly divided into five groups: HPS high-,mid-,low- dose group, rosiglitazone group and model group, additional 12 non transgenic db/m male mice with the same background were assigned into the normal group. The mice except for those of the normal group were intragatrically administered (i.g.) corresponding medicines for consecutive 8 weeks. Before treatment and every two weeks during the period of i.g. drugs, the body weight and blood glucose were detected. At the end of Week 8, HE staining was used to observe the pathological changes of left ventricular myocardial fibers, Masson staining was used to observe the degree of left ventricular myocardial collagen fibrosis, and Western blot, RT-PCR were used to measure the protein and mRNA expressions of TGF-β1 and Smad2, Smad3 in cardiac muscle tissue. Results Eight weeks after treatment, the blood glucose of rats in HPS high-, mid- dose group, and rosiglitazone group decreased significantly compared with model group (P<0.05). The sections of HE staining showed that the model rats’ myocardial cells structure became unclear and some of them were swollen and deformed obviously, even the nucleus disappeared. The sections of Masson staining showed that bright green collagen fibers were heaped up between myocardial cells and around blood vessels of the model rats. The protein and mRNA expressions of TGF-β1, Smad2 and Smad3 had no significant difference between model group and HPS low-dose group (P>0.05), but the other groups decreased significantly compared with the model group (P<0.05), especially the HPS high-dose group or rosiglitazone group was superior to mid-dose group (P<0.05). Conclusion The effect of HPS against myocardial fibrosis in db/db mice with DCM to slow down the progression of DCM was proved, and its potential mechanism may be related to inhibitation of TGF-β1/Smads signaling pathway.

Key words: diabetic cardiomyopathy, hedysarum polybotrys polysacchcaide, TGF-β1/Smads signaling pathway, db/db mice

CLC Number: 

  • R285.5