主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (1): 48-51.doi: 10.3969/j.issn.1006-2157.2017.01.010

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Effect of acupuncture at guānyuán (CV4) on skin temperature at bilateral sānyīnjīao (SP6) and on TXB2/6-keto-PGF1a of dysmenorrhea rats with coagulation cold pattern*

LUO Li1,2, REN Xiaoxuan1,3,GUO Mengwei1,3, ZHAO Yafang1,3, LI Xiaohong1,4#,JI Bo1,3,4,ZHANG Lufen1,3, ZHU Jiang1,4   

  1. 1 School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029;
    2 Department of Rehabilitation Medicine, the Affiliated Hospital of Southwest Medical University;
    3 Level-III Laboratory of Acupuncture Biological under State Administration of Traditional Chinese Medicine of China;
    4 Key Unit of State Administration of TCM: Evaluation of Characteristic Acupuncture Therapy
  • Received:2016-09-02 Online:2017-01-30 Published:2017-01-30

Abstract: Objective To observe the effect of acupuncture at guānyuán (CV4) on the skin temperature of bilateral sānyīnjīao (SP6) of dysmenorrhea rats with coagulation cold pattern, and to explore the potential mechanism related to TXB2/6-keto-PGF1a. Methods Rats were randomly divided into saline control group, dysmenorrhea rats with coagulation cold pattern group (model group) and needling at CV4 group (acupuncture group). The dysmenorrhea rat model with coagulation cold model was induced by whole body freezing combined with injection of estradiol benzoate and oxytocin. The infrared thermal imaging technology was used to detect the skin temperature at bilateral SP6 at the time point of 0, 5, 10, 20, 30, 40, 50, 60 minutes. Meanwhile, the serum thromboxane B2 (TXB2) and 6-keto-prostaglandin1a (6-keto-PGF1a) were measured by using ELISA. Results The skin temperature of left or right SP6 in saline control group showed balance at baseline and each time point (P>0.05). The model rats’ skin temperature of left or right SP6 was out of balance at the time points from 5 to 60 minutes (P<0.05 or P<0.01). After the treatment of acupuncture at CV4, the temperatures began to achieve balance between left and right SP6 from the time point of 40 minute (P>0.05). Compared with the saline control group, the serum TXB2/6-keto-PGF1a in model group significantly increased (P<0.05), and after needling at CV4, the serum TXB2/6-keto-PGF1a significantly decreased (P<0.05). Conclusion The significant effect of needling at CV4 against the imbalance of bilateral skin temperature of SP6 of dysmenorrhea rats with coagulation cold pattern would appear 10 to 40 minutes after needling. The mechanism may be related to the regulation of imbalance of vasomotor substances TXB2/6-keto-PGF1a.

Key words: dysmenorrheal model with coagulation cold pattern, needling at guānyuán (CV4), temperature of sānyīnjīao (SP6), infrared thermal imaging, TXB2/6-keto-PGF1a , rats

CLC Number: 

  • R246.3