主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (2): 125-129.doi: 10.3969/j.issn.1006-2157.2017.02.008

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Effect of Huoxue Jiedu Recipe on Hippo signaling pathway in diabetic rat retina *

LYU Tiantian, WANG Hongliang, XING Wei, WU Yan, WANG Wei, Zhang Zijian, HAN Jing#   

  1. Beijing University of Chinese Medicine, Beijing 100029
  • Received:2016-09-10 Online:2017-02-28 Published:2017-02-28

Abstract: Objective To observe the effect of Huoxue Jiedu Recipe(HJR), a Chinese medicine compound, on Hippo signaling pathway in diabetic rat retina. Methods A total 24 healthy SD rats were fasted for 12 h and administered intraperitoneally streptozotocin (65 mg/kg) once to induce diabetic model. After 20 weeks, the diabetic rats were randomly divided into model group and HJR group. And 10 more healthy rats were assigned into normal group with administration intragastrical saline. Each group was intervened once daily for consecutive 12 weeks. The levels of phosphorylated mammalian sterile 20-like kinase(P-MST), large tumor suppressor gene 1 (Lats1), phosphorylated Yes-associated protein(P-YAP), tafazzin (TAZ), TEA domain family member(TEAD) protein in retinal tissue were detected by immunohistochemical method and Western blot. Results Compared with the normal group, the protein expression of P-MST and P-YAP were decreased in the model group. While compared with the model group, the protein expression of P-MST and P-YAP significantly increased in HJR group. And Western blot results showed that protein expression of Lats1, TAZ and TEAD in model group were higher than those of normal group, and HJR decreased the protein expression of Lats1, TAZ and TEAD. Conclusion The effect of Huoxue Jiedu Recipe on diabetic retinopathy may be via regulating Hippo signaling pathway.

Key words: Huoxue Jiedu Recipe, diabetic retinopathy, Hippo signaling pathway, rats

CLC Number: 

  • R285.5