主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (2): 143-147.doi: 10.3969/j.issn.1006-2157.2017.02.011

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Effect of Feixian Fang extract on mRNA expression of VEGF, VEGFR2, PAI-1 and VCAM-1 in microvascular endothelial cells of rats with pulmonary fibrosis: an in vitro study*

WANG Chen1, WANG Zijuan1, ZHANG Xiaomei2#,YIN Ting2, LIU Zhe1, QIN Huihui1, ZHANG Yu-ting1, GU Xiaofeng1, YU Xiaolin1   

  1. 1 Beijing University of Chinese Medicine, Beijing 100029;
    2 Dongfang Hospital, Beijing University of Chinese Medicine
  • Received:2016-09-12 Online:2017-02-28 Published:2017-02-28

Abstract: Objective To investigate the effect of Feixian Fang extract (FXF) on the expression of vascular endothelial cell growth factor(VEGF), vascular endothelial cell growth factor receptor 2 (VEGFR 2), vascular cell adhesion molecule-1(PAI-1) andplasminogen activator inhibitor-1 (VCAM-1) in pulmonary microvascular endothelial cells in vitro, so as to explore the mechanism of intervention of FXF on microvascular angiogenesis of pulmonary fibrosis. Methods The lung microvascular endothelial cells which were isolated from pulmonary fibrosis model rats were equally divided into model group, losartan group, prednisone group, FXF high-, mid-, and low- dose group. The cells of model group were cultured in DMEM containing 20% fetal bovine serum, those of losartan group were cultured in DMEM containing 20% fetal bovine serum with 10 g/L losartan, those of prednisone group were cultured in DMEM containing 20% fetal bovine serum with 5 mg/L prednisone, and those of FXF groups were cultured in DMEM containing 20% fetal bovine serum with 100, 60, or 20 mg/L Feixian Fang extract. All the cells were cultured for 24 h. Then the mRNA expression of VEGF, VEGFR2, PAI-1 and VCAM-1 were detected by using method of double chain chimeric fluorescent dye SYBR Green real-time fluorescence quantitative detection. Results Campared with the model group, VEGF mRNA expression decreased significantly in all FXF group, while there was no difference of VEGFR2; PAI-1 mRNA was downregulated in FXF high-dose group; VCAM-1 mRNA expression decreased significantly in both FXF high-dose group and FXF low-dose group(P<0.05).Conclusion The effect of FXF against pulmonary fibrosis via the way of improving the blood coagulation and fibrinolysis leading to inhabitation of angiogenesis, due to downregulating the mRNA expression of VEGF, PAI-1 and VCAM-1 in the lung microvascular endothelial cells.

Key words: Feixian Fang extract, pulmonary interstitial fibrosis microvascular endothelial cells, vascular endothelial cell growth factor, vascular endothelial cell growth factor receptor 2, plasminogen activator inhibitor-1 , vascular cell adhesion molecule-1, rats

CLC Number: 

  • R285.5