主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (3): 193-199.doi: 10.3969/j.issn.1006-2157.2017.03.004

Previous Articles     Next Articles

Protective effect of Rooibos on cerebral ischemia-reperfusion injury in mice*

MA Qing, LI Ya, TANG Minke#   

  1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102
  • Received:2016-01-09 Online:2017-03-30 Published:2017-03-30

Abstract: Objective To observe the protective effect of Rooibos (endemic plant in the South Africa) in mice with cerebral ischemia-reperfusion injury (IRI). Methods All mice were divided into control group, IRI group, low-dose Rooibos group (low-dose group, 2.8 g/kg), mid-dose Rooibos group (mid-dose group, 5.6 g/kg) and high-dose Rooibos group (high-dose group, 11.2 g/kg). Before modeling, 3 medicated groups were orally given Rooibos once a day for 7 d, and control group and IRI group were given isometric distilled water. After one week, the model was established by using cerebral middle cerebral artery occlusion (MCAO), and then all groups were continuously given Rooibos or distilled water for 7 d. The influence of Rooibos on cerebral infarction volume was observed after TTC staining. The influence of Rooibos on movement, cognition and sense was observed after neurologic score test, balance beam test, Morris water maze (MWM) test and corner test. The influence of Rooibos on redox state after cerebral injury was observed after detecting the activities of superoxide dismutase (SOD) and glutathione peroxidease (GSH-PX), and content of malondialdehyde (MDA). The influence of Rooibos on inflammatory state after cerebral injury was observed after detecting the brain contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and intercellular adhesion molecule-1 (ICAM-1). Results The cerebral infarction volume decreased significantly (P<0.05), scores of balance beam test decreased significantly (P<0.01), times from corner to non-injury side decreased significantly (P<0.01), and contents of TNF-α, IL-1β and ICAM-1 decreased significantly (P<0.01 or P<0.05) in 3 Rooibos groups compared with IRI group. Conclusion Rooibos has certain protective effect in mice with cerebral IRI, and the effect may be related to the decrease of inflammation factors in brain tissue.

Key words: Rooibos, cerebral ischemia, neurologic score, oxidative stress, inflammation factors, mice

CLC Number: 

  • R285.5