主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (3): 208-213.doi: 10.3969/j.issn.1006-2157.2017.03.006

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Influence of surrounding-hoop method under guidance of defended field theory on expressions of plasma HMGB1 and LXA4 in rats with skin abscess*

LIU Tingting1, ZHU Chaojun2, XU Qiang2, ZHANG Zhaohui2#, ZHANG Yang3, WANG Wanying1, JI Xiaona1, GUO Yue3, ZHAO Likun3   

  1. 1 Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine, Tianjin 300193;
    2 Tianjin Hospital;
    3 Graduate School of Tianjin University of Traditional Chinese Medicine
  • Received:2016-09-03 Online:2017-03-30 Published:2017-03-30

Abstract: Objective To observe the influence of surrounding-hoop method (Hongzhongxiao Tincture) under guidance of defended field theory on expressions of plasma high mobility group box-1 protein (HMGB1) and lipoxin A4 (LXA4) in rats with skin abscess. Methods All rats (n=40) were randomly divided into blank group, model group, control group, pretreatment group and treatment group. Except of blank group, other 4 groups were given hypodermic injection of 1 mL Staphylococcus aureus (3-5×10109 CFU/mL) for establishing the model of skin abscess. The model group was given normal saline dressing, control group was given Mupirocin Ointment dressing, pretreatment group was given 2 mL Hongzhongxiao Tincture dressing 4 h after injection of Staphylococcus aureus, and treatment group was given 2 mL Hongzhongxiao Tincture dressing over 1 cm around abscess once a day. The blood samples (2 mL) were collected respectively after 3 d, 7 d, 14 d and 18 d. The expressions of HMGB1 and LXA4 were detected by using enzyme-linked immunosorbent assay (ELISA). Results The content of HMGB1 was lower in model group compared with blank group (P<0.01), and higher in pretreatment group and treatment group compared with model group (P<0.01 or P<0.05) 3 d after treatment. The content of LXA4 increased in model group, control group and treatment group compared with blank group (P<0.01 or P<0.05) 3 d after treatment. The content of LXA4 was lower in pretreatment group than that in model group (P<0.01). The content of LXA4 increased in control group and blank group compared with model group (P<0.05) 18 d after treatment. Conclusion Early surrounding-hoop method (Hongzhongxiao Tincture) can improve the expression of plasma LXA4 through taking part in early inflammation inhibition, and shortening inflammatory progress at the same time, promoting HMGB1 release at early treatment stage and inhibiting its release and/or stopping its proinflammatory activity at middle and late treatment stages for improving effectively the formation of defended field.

Key words: defended field theory, surrounding-hoop method, skin abscess, Hongzhongxiao Tincture, high mobility group box-1 protein, lipoxin A4, rats

CLC Number: 

  • R285.5