主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (3): 219-225.doi: 10.3969/j.issn.1006-2157.2017.03.008

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Influence of Tongluo Baoshen Fang on indexes of renal endoplasmic reticulum stress-P-IRE1α and GRP78 in rats with diabetes*

ZHANG Jie, LI Jianmin#, RONG Xiaolong, LIU Xiuping, ZHOU Guomin, HAN Xue, ZHOU Yu, HE Hua, SONG Yue   

  1. Department of Nephropathy, Beijing Hospital of Integrated Traditional Chinese and Western Medicine, Beijing 100039
  • Received:2016-09-28 Online:2017-03-30 Published:2017-03-30

Abstract: Objective To discuss the influence of Tongluo Baoshen Fufang (TBF) and its disassembled formulas on P-IRE1α, promoter of endoplasmic reticulum stress (ERS) and GRP78, molecular chaperone, in diabetic rats with hyperglycemic kidney injury induced by streptozocin (STZ). Methods Male SD rats were randomly divided into normal group and modeling group. The model of diabetes was established by applying intraperitoneal injection of STZ in model group. After successfully modeling, the modeling group was randomly divided into model group, valsartan group(8.93 mg/kg), TBF group(19.43 g/kg), Tongluo Baoshen Buxu group (TBB group,6.92 g/kg) and Tongluo Baoshen Jiedu group (TBJ group,12.5 g/kg). The general conditions of rat daily activities were observed in all groups. The expressions of p-IRE1α and GRP78 were analyzed semi-quantitatively by using immunohistochemistry and IPP software after 6 weeks and 16 weeks. Results P-IRE1α and GRP78 mainly existed in the cytoplasm of proximal and distal renal tubule cells and glomerular cells observed by light microscope. The results of semi-quantitative immunohistochemistry and IPP software showed that the expressions of P-IRE1α and GRP78 increased in model group compared with normal group after 6 weeks and 16 weeks. Compared with model group, the expressions of P-IRE1α and GRP78 decreased in valsartan group, TBF group, TBB group and TBJ group (P<0.05) after 6 weeks and 16 weeks. The comparison among valsartan group, TBF group, TBB group and TBJ group did not show difference(P>0.05). Conclusion The rat model of kidney injury induced by hyperglycemia has ERS activation. TBF and its disassembled formulas can take play a renal protective role through inhibiting ERS. It can be speculated that the central pathogenesis of diabetic hyperglycemic kidney injury is deficiency of the root and excess of the tip in Chinese medicine.

Key words: endoplasmic reticulum stress, diabetic nephropathy, Tongluo Baoshen Fang, immunohistochemistry, rats

CLC Number: 

  • R285.5