主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (3): 247-253.doi: 10.3969/j.issn.1006-2157.2017.03.013

Previous Articles     Next Articles

Regulation effect of electro-acupuncture on expression of endometrium phosphorylation insulin-like growth factor-1 receptor and its signal transduction pathways in mice with controlled ovarian hyperstimulation*

ZHU Shuxiu1, LIU Jie2#, WANG Fang1, LIU Wei1, LI Changlei1, FU Qiang1, ZHANG Wejuan1   

  1. 1 Department of Traditional Chinese Medicine, College of Medical Sciences, Jianghan University, Hubei 430065;
    2 Center for Reproductive Medicine, Maternal and Child Health Hospital of Hubei Province
  • Received:2016-09-26 Online:2017-03-30 Published:2017-03-30

Abstract: Objective To observe the influence of electro-acupuncture (EA) on expression of endometrium phosphorylation insulin-like growth factor-1 receptor (p-IGF-1R), and signal transduction pathways of MAPK and PI3K in mice with controlled ovarian hyperstimulation (COH), and discuss the mechanism of EA improving embryo implantation. Methods Female Kunming mice were randomly divided into natural cycle group, COH group, EA group, EA+BMS-536924 group, EA+LY-294002 group and EA+PD-98059 group (each n=20), and mice were divide again into donor mice and recipient mice. The model was established by applying controlled hyperstimulation program, and given EA in Guanyuan (RN3), Zhongji (RN4) and Sanyinjiao (SP6) at the day of injecting human chorionic gonadotropin (HCG) once a day until stopping collecting samples. The fertilized ovums were collected from donor mice and cultured in vitro, and then transplanted into the uterus of recipient mice. The clinical pregnancy rate and site number of embryo implantation were observed. The expressions of endometrial IGF-1R, p-IGF-1R, phosphorylation protein kinase (p-Akt) and phosphorylation extracellular regulated protein kinases1/2 (p-ERK1/2) were detected by using Western Blot. Results The clinical pregnancy rate, site number of embryo implantation and expressions of endometrial IGF-1R, p-IGF-1R, p-Akt and p-ERK1/2 were all lower in COH group than those in natural cycle group (P<0.05 or P<0.01). All indexes were higher in EA group, EA+LY-294002 group and EA+PD-98059 group than those in COH group (P<0.05 or P<0.01). But the clinical pregnancy rate, site number of embryo implantation had no significant improvement in EA+BMS-536924 group, and expression of p-IGF-1R was significantly lower than that in natural cycle group (P<0.01). The comparison among EA+BMS-536924 group, EA+LY-294002 group and EA+PD-98059 group showed that the expression of p-Akt was higher in EA+PD-98059 group, and expression of p-ERK1/2 was higher in EA+LY-294002 group (P<0.01). Conclusion Embryo implantation can be improved by EA in COH mice, which may be related to EA can up-regulate the expression of p-IGF-1R and signal transduction pathways of PI3K/Akt and MAPK/ERK1/2.

Key words: controlled ovarian hyperstimulation, electro-acupuncture, phosphorylation insulin-like growth factor-1 receptor, phosphorylation protein kinase, phosphorylation extracellular regulated protein kinases1/2, mice

CLC Number: 

  • R245