主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (4): 302-307.doi: 10.3969/j.issn.1006-2157.2017.04.008

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Effects of Xiaosheng San on goblet cells of dry eyes in mice: a laboratory study*

QI Yixin, XIE Like#, HAO Xiaofeng, ZHANG Zhifang, HOU Le, XIAO Wenzheng, SUN Huilan, MING Jing   

  1. Ophthalmology Hospital, China Academy of Chinese Medical Sciences, Beijing 100040
  • Received:2016-12-31 Online:2017-04-30 Published:2017-04-30

Abstract: Objective To study the effects of Xiaosheng San(XSS, Xiaosheng Granules) on goblet cells of dry eyes in mice and evaluate its mechanism.Methods 180 male C57BL/6 mice were divided into six groups: normal control (group A), model (group B), saline (group C), XSS low-dose (group D), XSS medium-dose (group E) and high-dose (group F) groups. Dry eye model was established by injecting scopolamine subcutaneously at the base of rear legs in all groups except normal control group. Schirmer I test (SIT), tear break-up time (BUT),corneal fluorescent (FL) staining, and the number of conjunctival goblet cells before and after modeling, and at four weeks after intervention were measured.Results BUT, SIT,and number of goblet cells in group B, C, D, E,and F were significantly decreased after scopolamine treatment(P<0.05), while no significant difference (P>0.05) was found in group A.FL were significantly increased after model establishment (P<0.05).Medium- and high-dose XSS significantly improved SIT, BUT and the number of goblet cells in group E and F (P<0.05). No such changes were observed in group A, B and C (P>0.05). Conclusion Subcutaneous injecfion of scopolamine could effectively establish dry eye model in mice. Xiaosheng Granules could increase tear secretion, prolong tear break-up time, improve stability, inhibit apoptosis of goblet cells and reduce injury to eye surface.

Key words: xiaoshengsan, dry eye, goblet cells, mice

CLC Number: 

  • R285.5