主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (5): 392-398.doi: 10.3969/j.issn.1006-2157.2017.05.007

Previous Articles     Next Articles

Regulating effect of Zuogui Jiangtang Jieyu Fang on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in simulated condition of diabetes complicating depression*

LIU Jian1, WANG Yuhong2#, HAN Yuanshan1, XU Yalan3, LIU Lin1, YANG Hui1, LI Wei1, XIANG Yun2   

  1. 1 First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan 410007, China;
    2 State Key Laboratory and Breeding Base of Power and Innovative Medicine of Chinese Pharmacy, Hunan 410208, China;
    3 Changde Vocational Technical College, Hunan 415003, China
  • Received:2016-10-20 Online:2017-05-30 Published:2017-05-30
  • Supported by:
    Project sponsored by National Natural Science Foundation (No.81373578, No.81573965), Youth Fund Project sponsored by National Natural Science Foundation (No.81403379, No.81603604), Natural Science Foundation of Hunan Province (No.2017JJ3241)

Abstract: Objective To investigate the influence of Zuogui Jiangtang Jieyu Fang (ZJJF) on hippocampus neuron synaptic plasticity related proteins-CaMKⅡ and SynGap in rats simulated diabetes complicating depression DD. Methods The hippocampus neurons were primitively isolated, purified and cultured in SD rats. The DD model was established by applying intervention of high glucose and cortisone. The cultured hippocampus neurons were randomly divided into normal group, model group, medicated serum of ZJJF (ZJJF group), medicated serum of positive drug (fluoxetine and metform) group (positive drug group) and blank group. The damages of hippocampus neurons were detected by using Nissl’s staining in all groups, and expressions of CaMKII and SynGap were detected by using immunofluorescence staining and high-content analysis (HCA) after drug intervervention for 18 d. Results Compared with normal group and blank group, the quantity of Nissl bodies decreased significantly, neural network and dentritic spines ruptured or disappeared, and expressions of CaMKII and SynGap were significantly down-regulated in model group (P<0.01). Compared with model group, the quantity of Nissl bodies, neural network and dentritic spines, and synaptic connection were significantly recovered, and expressions of CaMKII and SynGap were un-regulated in positive drug group and ZJJF group (P<0.01 or P<0.05). Conclusion ZJJF had significant regulative effect on CaMKII and SynGap in simulated condition of diabetes complicating depression, which may be one of important mechanisms of ZJJF antagonizing cellular damage and neural degeneration.

Key words: diabetes complicating depression, cell culture, hippocampus neurons, Zuogui Jiangtang Jieyu Fang, calmodulin dependent protein kinase II, SynGap, rats

CLC Number: 

  • R285.5