主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (5): 399-404.doi: 10.3969/j.issn.1006-2157.2017.05.008

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Influence of Mongolia medicine-Garidi San on Th1 and Th17 in rats with ulcerative colitis*

WANG Lin1,2, PEI Ling-yan1, KE Yushi1, CUI Jian1, LI Shucun1#   

  1. 1 Institute of Traditional Chinese Minority Medicine, Minzu University of China, Beijing 100081, China;
    2 Department of Histology and Embryology, Xinxiang Medical University, Henan 453003, China
  • Received:2016-10-25 Online:2017-05-30 Published:2017-05-30
  • Supported by:
    Project sponsored by National Natural Science Foundation (No.81273872)

Abstract: Objective To discuss the influence of Mongolia medicine-Garidi San on helper T cell 1 (Th1) and helper T cell 17 (Th17) in rats with ulcerative colitis (UC). Methods All rats were randomly divided into normal group, model group, low-dose Garidi San group (low-dose group), mid-dose Garidi San group (mid-dose group), high-dose Garidi San group (high-dose group), sulfasalazine group (SSZ group) and Bupi Yichang Wan group. The UC model was induced and established by using immune method and TNBS. Garidi San was orally given to, respectively, low-dose group (1.76 g/kg), mid-dose group (3.51 g/kg) and high-dose group (7.02 g/kg), SSZ was given to SSZ group (3.60 g/kg), and Bupi Yichang Wan was given to Bupi Yichang Wan group (1.62 g/kg). After 4 weeks the samples of peripheral blood were collected from all groups. The ratio of Th1 to Th17 (Th1/Th17) in peripheral blood was observed by using flow cytometry, and levels of PB transforming growth factor β (TGF-β), interleukin-6 (IL-6), interleukin-17 (IL-17) and interferon-γ (IFN-γ) were detected by using enzyme-linked immunosorbent assay (ELISA). Results The level of TGF-β was decreased significantly in mid-dose group, low-dose group and SSZ group. The levels of serum IL-6 and IL-17 were decreased significantly(P<0.05), level of IFN-γ was increased (P<0.05), and Th1/Th17 was increased (P<0.05) in all medication groups. Conclusion Garidi San can regulate the differentiation direction of helper T cell 0, and recover the balance of Th1/Th17 in UC rats, which may be one of mechanisms of Garidi San curing UC.

Key words: Garidi San, ulcerative colitis, helper T cell 1, helper T cell 17, rats

CLC Number: 

  • R291.2