主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (5): 420-427.doi: 10.3969/j.issn.1006-2157.2017.05.011

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Relevant analysis on quality representation based on nucleosides in Aurea helianthus*

KONG Jing1, HE Jing1, TAN Li1, SHEN Lifeng2, LI Qian2, ZHANG Yuanyuan1, LV Shan1, WU Qiuhong1, CHEN Mengjie1, LIU Lu1, LI Wenxia1, LU Dongbo1, SHI Renbing1,3, JIANG Yanyan1,3#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 School of Chinese Medicine, Capital University of Medical Sciences, Beijing 100069, China;
    3 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 100102, China
  • Received:2017-01-27 Online:2017-05-30 Published:2017-05-30
  • Supported by:
    Project sporsored by National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2012BAI29B06), Joint project of Research Base of Beijing Municipal Education Commission

Abstract: Objective To establish a method of high performance liquid chromatography (HPLC) for simultaneously determining of 7 nucleoside (uracil, cytidine, uridine, adenine, guanosine, adenosine and 2'-deoxyadenosine) in Aurea helianthus, and conduct the relevant analysis and review on quality of Aurea helianthus. Methods HPLC-PDA was applied for determining 7 nucleosides in Aurea helianthus. The quality representation of 11 batches of Aurea helianthus was carried out and representation results were given relevant analysis based on the content of index components and index component adducts from 7 nucleosides. Results The linear relations of 7 nucleosides were good, and precision, stability, repeatability and sample recoveries were all accorded with the requirements of methodology determination. The content of 7 nucleosides in 11 batches of Aurea helianthus was determined by using the established method. Taking batch lot 1 of Aurea helianthus as criterion material, the content of index components was higher in batch lot 2, 6, 9, 10 and 4, and relevancy was the highest among criterion Aurea helianthus and batch lot 6, 9, 8, 4, 3 and 11. The comprehensive review showed that excellent degrees of batch lot 1, 2, 6, 9 and 4 were in the lead. Conclusion The established method for determining content of nucleosides in Aurea helianthus and correlation analysis mode of quality representation can analyze overall quality relevancy and application validity, which provides the reliable evidence for quality control and review of Aurea helianthus.

Key words: Aurea helianthus, nucleosides, high performance liquid chromatography, content determination, relevant analysis, quality review

CLC Number: 

  • R284.2