主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (6): 492-497.doi: 10.3969/j.issn.1006-2157.2017.06.008

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Study on the specificity of Tau protein phosphorylation sites in three typical patterns of senile dementia based on the same disease with different patterns*

GUO Bin, GUO Lei#, HAN Cheng, QIN Yali, SHI Weibin, HOU Jiangqi   

  1. Shanxi University of Traditional Chinese Medicine, Shanxi, 030024, China
  • Received:2016-12-21 Online:2017-06-10 Published:2017-06-10

Abstract: Objective To observe Tau protein phosphorylation in rats with three typical patterns of senile dementia, analyzing and summarizing the specificity of phosphorylation. Methods We ligated and dissected the left and right carotid arteries at one week’s interval with intraperitoneal injection of D-galactose to establish senile dementia model in rats. Those rats then received the intervention of excessive sexual and physical exertion, high-fat feeding milk and low temperature environment respectively to establish the rat models of senile dementia with three different patterns (group K: kidney deficiency and essence loss pattern; group P: retention of phlegm and turbid substance in orifices pattern; group C: congealing cold and blood stasis pattern). Blood lipid, blood rheology and serum testosterone were measured to identify whether those model was built successfully. Different positions of Tau phosphorylation sites were measured with Western blot method. Results In the Tau protein proline-rich region, compared with the diseased groups, phosphorylation level increased significantly at the sites of 205 and 231 in group P. This increase was also present at sites of 181, 205, and 231 in group K and 205, 231 and 262 in group C. At the C-terminal region, compared with the diseased group, phosphorylation level of P group and K group were significantly increased at the sites of 404, and the increase of group C was the highest among those groups. Conclusion There is specificity at the Tau protein phosphorylation sites in each typical senile dementia pattern. This study may reveal the scientific rationale for the presence of different patterns in the same disease.

Key words: senile dementia, syndrome, combination of disease and pattern, Tau protein phosphorylation sites, rats

CLC Number: 

  • R285.5