主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (7): 558-563.doi: 10.3969/j.issn.1006-2157.2017.07.006

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Influences of Banxia Xiexin Tang and guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice*

SHENG Ning1, HE Yingkun1,2, FENG Huili1, DONG Yunfang1, FAN Hui1,3, DANG Huizi1,4, REN Ying1, YANG Jinduo, WANG Pengwen1#   

  1. 1 Education Ministry and Beijing Key Laboratory of Chinese Internal Medicine, Dongzhimen Hospital, Beijing University of Chinese Medicine, Beijing 100700, China;
    2 Hebei General Hospital, Hebei 050051, China;
    3 Beijing Chaoyang Integrative Medicine-Emergency Medical Center, Beijing 100022, China;
    4 Beijing Huiming Hospital, Beijing 100053, China
  • Received:2017-01-15 Online:2017-07-10 Published:2017-07-10
  • Supported by:
    National Natural Science Foundation of China (No. 81573927), Scientific Research and Graduate Training Joint Project of Beijing Municipal Education Commission (No. 2016)

Abstract: Objective To detect the influences of Banxia Xiexin Tang (Pinellia Heart-Draining Decoction) and guanosine on learning and memory in APPswe/PS1dE9 double transgenic mice (6 months old) by applying step-down test and Morris water maze (MWM). Methods The mice were randomly divided into model group (given 0.5% CMC intragastrically), Western drug control group [WD group, given donepezil in 0.92 mg/(kg·d) intragastrically], Banxia Xiexin Tang group [BXT group, given Banxia Xiexin Tang in 650 mg/(kg·d) intragastrically], Chinese medicinal control group [CM group, given curcumin in 200 mg/(kg·d)] and guanosine group (given 0.5%CMC intragastrically, each n=15). The guanosine group was given intraperitoneal injection of guanosine [16 mg/(kg·d)], and other groups were given intraperitoneal injection of isopyknic normal saline (NS). The brood wide type C57BL/6J mice (n=15) were set into normal group and given 0.5%CMC intragastrically and isopyknic NS. All groups were given corresponding interventions when mice were 3 months old. The ethological detection was conducted by using step-down test and MWM in al groups after 3 m. Results The results of step-down test showed that latency was significantly shorter in model group than that in normal group (P<0.01), and was significantly longer in all medicinal intervention groups than that in model group (P<0.01). The results of MWM showed that escape latency was shortened, swimming distance showed decline trend with slight fluctuation in all groups along with days increasing. The results of MWM showed that in spatial probe test, the time of targeting quadrant was significantly shorter in model group than that in normal group (P<0.01), and was longer in WD group, CM group and BXT group than that in model group (P<0.05). Conclusion Banxia Xiexin Tang has an ameliorative effect on early learning and memory disorder, and guanosine can improve the ability of memory retaining in APPswe/PS1dE9 double transgenic mice.

Key words: guanosine, Banxia Xiexin Tang (Pinellia Heart-Draining Decoction), Alzheimer’s disease, ethology, mice

CLC Number: 

  • R285.5