主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (7): 572-577.doi: 10.3969/j.issn.1006-2157.2017.07.008

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Influences of baldrinal on gastrointestinal sensitivity and expressions of 5-HIAA and relevant receptors of 5-HT in rats with irritable bowel syndrome*

TAO Siyu1, XIAO Congrui2, WANG Jing2, ZHANG Shujing1, GAO Yushan1, MU Fangyuan1, WANG Yicheng1, YAN Xingli1#   

  1. 1 School of Preclinical Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China
  • Received:2017-02-17 Online:2017-07-10 Published:2017-07-10
  • Supported by:
    National Natural Science Foundation of China (No. 81374044)

Abstract: Objective To investigate the effect of baldrinal on irritable bowel syndrome (IBS) induced by chronic-acute stress and its influences on the protein expressions of 5-hydroxyindoleacetic acid (5-HIAA) and relevant receptors of 5-hydroxytryptamine (5-HT) in rats. Methods SD rats were randomly divided into control group, model group, high-dose baldrinal group (0.6 mg/kg), mid-dose baldrinal group (0.3 mg/kg), low-dose baldrinal group (0.15 mg/kg) and pinaverium group (25 mg/kg). The rat model of IBS was established by using chronic-acute stress combined with independent feeding for observing the influence of baldrinal on the scores of abdominal withdraw reflection (AWR). The content of colonic 5-HIAA was detected by using high performance liquid chromatography (HPLC), and influences of baldrinal on protein expressions of colonic 5-HT1A, 5-HT3A and 5-HTR4 were detected by using Western blotting. Results The scores of AWR content of colonic 5-HIAA were decreased in high-dose baldrinal group, mid-dose baldrinal group and low-dose baldrinal group compared with model group (P<0.05). The protein expressions of colonic 5-HT1A, 5-HT3A and 5-HTR4 were regulated in high-dose baldrinal group, mid-dose baldrinal group and low-dose baldrinal group. Conclusion Baldrinal has significant inhibitory effect on gastrointestinal hyperfunction in rat model of IBS and reduce viscera sensitivity, which indicates that baldrinal can relieve IBS symptoms. The effective mechanism may be related to the regulation effect of baldrinal on 5-HIAA contents and protein expressions of relevant receptors of 5-HT.

Key words: baldrinal, irritable bowel syndrome, 5-hydroxyindoleacetic acid, receptors of 5-hydroxytryptamine

CLC Number: 

  • R285.5