主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (8): 646-652.doi: 10.3969/j.issn.1006-2157.2017.08.006

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Effects of Yiqi Huoxue Fang on necrosis and apoptosis of myocardial cell in rats with myocardial infarction*

CHEN Xi,GUO Shuwen#,WU Jiangong,LIU Wenchen,ZHANG Lu,SU Zhaoduo,WU Jiani,LI Fanghe   

  1. Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2017-03-07 Online:2017-08-10 Published:2017-08-10
  • Supported by:
    National Natural Science Foundation of China(No.81473552)

Abstract: Objective To explore the protective effects of Yiqi Huoxue Fang(Qi-boosting Blood-activating Formula, YQHXF)on rat myocardium with myocardial infarction and its possible mechanism by observing the effects of YQHXF on necrosis and apoptosis of myocardial cell necrosis.Methods Male SD rats were randomly divided into the sham-surgery group, model group,YQHXF group, and perindopril group.Animal model of myocardial infarction was built by ligating coronary artery on the left anterior descending branch.Subsequently, YQHXF group received intragastric administration of Yiqi Huoxue Fang (compound medicine, 21 g/kg) once per day, and perindopril group was given perindopril (0.4 mg/kg) once a day;Sham-surgery group and model group received sterile distilled water without drug intervention.The blood in abdominal aorta was drawn, and the tissue on the peripheral area of the myocardial infarction was taken at two fixed time points (on the 7th and 28th day) after administration.Ultrasonic echocardiography was used to detect changes on cardiac morphology and function in rats: left ventricular ejection fraction (LVEF), left ventricular short axis ratio (LVFS), left ventricular end systolic diameter (LVIDs), left ventricular end-diastolic diameter (LVIDd); serum superoxide dismutase (SOD), glutathione peroxidase (gsh-px), creatine kinase (CK), lactate dehydrogenase (LDH), aspertate aminotransferase (AST), and troponin (cTnT) were measured with ELISA method. Protein imprinting method (Western Blot) was used to measure cytochrome C (CytC) and protein expression of cysteine aspartic acid and protease-3 (caspase-3) in the border zone of infarcted area. Results On the 7th day, compared with the model group, LVEF and LVFS in YQHXF group and perindopril group decreased without statistical significance(P>0.05). On the 28th day, compared with the model group, LVEF and LVFS in YQHXF group and perindopril group increased significantly(P>0.05).LVIDs and LVIDd decreased markedly(P<0.05); LVIDd in perindopril group decreased sharply (P<0.05), and decrease presented on LVIDs without statistical significace (P>0.05). On the 7th and 28th day, compared with the model group, CK-MB, LDH, AST, cTnT in YQHXF group and perindopril group decreased significantly (P<0.05), and SOD, GSH-PX increased significantly. On the 7th day,protein expression of cytC,caspase-3 decreased significantly in YQHXF group and perindopril group(P<0.05).Conclusion Administration of YQHXF at early stage can effectively improve myocardial infarction on cardiac structure and function in rats with myocardial infarction, improve oxidation resistance of myocardial cell, and inhibit myocardial apoptosis and necrosis.

Key words: myocardial infarction, myocardial markers, antioxidant enzymes, Yiqi Huoxue Fang, apoptosis, rats

CLC Number: 

  • R285.5