主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (8): 684-692.doi: 10.3969/j.issn.1006-2157.2017.08.011

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Quality representation and correlation analysis of Shanzha based on phenolic characteristic spectrum of medical system*

ZHAO Bo1, TIAN Hengkang2, SUN Daohan1, SHEN Lifeng3, KONG Jing1, LU Dongbo1, LI Wenxia1, WU Qiuhog1, LIU Lu1, LI Qian3, ZHANG Yuanyuan1, LYU Shan1,CHEN Mengjie1, SHI Renbing1,4#   

  1. 1 Key Unit of Exploring Effective Substances of Classical and Famous Formulas of State Administration of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 Center of Drug Evaluation, Beijing 100038, China;
    3 School of Chinese Medicine, Capital Medicinal University Beijing 100069, China;
    4 Quality Control Technology and Engineering Center of Chinese Medicine, Beijing Municipal Commission of Education, Beijing 100069, China
  • Received:2017-05-08 Online:2017-08-10 Published:2017-08-10
  • Supported by:
    National Sci-Tech Support Plan of the 12th Five-Year Plan (No.2012BAI29B06)

Abstract: Objective To establish quality representation and correlation analysis method based on the spectrum of medicinal system of Shanzha(Fructus Crataegi, Chinese hawthorn fruit),so as to evaluate quality of Shanzha decocting pieces effectively and accurately. Methods HPLC-PDA method was applied to characterize quality of spectrum of Shanzha medicinal system; quality of 11 batches of Shanzha decocting pieces were characterized based on the number and chemical type of peaks on characteristic spectrum.The content of characteristic index components, protocatechuic acid, epicatechin,chlorogenicacid, phenolic acids (represented by protocatechuic acid and chlorogenic acid) and flavonoids (represented by hyperoside and epicatechin) were characterized by indexes in characteristic spectrum. Correlation analysis of quality and quantity of Shanzha was then performed with reference to the standard reference pieces.Results As the reference substance, characteristic spectrum of batch 1 contains 13 characteristic peaks, 7 peaks of flavonoids and 6 peaks of phenolic acids.All characteristic peaks appeared on the chromatograms of 11 batches of Shanzha.Batch 5,10, 8, 2,6 had more effective index ingredients. After comprehensive evaluation,there was a high correlation between batch 6, 4, 3, 7, 8, 2 and standard reference pieces. The quality of batch 5, 10, 8, 2, 7, and 3 ranked on the top among all samples.Conclusion HPLC method to characterize quality and quantity of characteristic spectrum of 11 batches of Shanzha is simple and accurate. The evaluation mode of quantity and quality and their correlation based on Shanzha medical system, including systemic correlation and application validity, can evaluate the quality of hawthorn pieces effectively and accurately.

Key words: Shanzha, characteristic spectrum, quality evaluation, correlation analysis ,  ,  

CLC Number: 

  • R284.1