主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (9): 729-735.doi: 10.3969/j.issn.1006-2157.2017.09.005

• CONTENTS • Previous Articles     Next Articles

Influence of Pixu Fang 1 (Spleen-deficiency Formula 1) on protein and gene expressions of myosin light chain in rats with functional dyspepsia-spleen deficiency pattern*

LYU Lin1, WANG Fengyun1, TANG Xudong1#, YIN Xiaolan2, SHI Xiaoshuang2, DUAN Yuanzhi2   

  1. 1 Department of Gastroenterology, Beijing Institute of Spleen and Stomach Diseases, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, China;
    2 Graduate School, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2017-04-06 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    National Key Basic Research Development Plan (973 Plan, No.2013CB531703), National Natural Science Foundation of China (No.81503567), China Postdoctoral Science Foundation (No.2015M1227, 2016T90195)

Abstract: Objective To investigate the intervention of Pixu Fang 1 (Spleen-deficiency Formula 1) to the protein and mRNA expressions of myosin light chain (MLC) in gastric tissue in rats with functional dyspepsia (FD)-spleen deficiency pattern (SDP). Methods SD rats (n=60, aged 7 d) were randomly divided into normal group (n=10) and FD-SDP model group (n=50). The normal group was orally given 2% sugar solution and FD-SDP model group was given 0.1% iodoacetamide sugar solution in a dose of 0.2 mL each rat a day for 6 d. The FD-SDP model group was fed with normal forage until rats aged 6 weeks and additionally given modified multiple platform method (MMPM) continuously for 14 d. After modeling, FD-SDP model group was divided randomly into model group (distilled water, 10 mL/kg·d), domperidone group (3.125 mg/kg·d), low-dose Pixu Fang 1 group (low-dose group, 1.275 g/kg·d), mid-dose Pixu Fang 1 group (mid-dose group, 2.550 g/kg·d) and high-dose Pixu Fang 1 group (high-dose group, 5.100 g/kg·d, each n=10), and all groups were given orally corresponding medicinal for 14 d. The protein and mRNA expressions of MLC in gastric tissue were detected by using immunohistochemistry technique, Western blotting and reverse transcription-polymerase chain reaction (RT-PCR). Results Compared with normal group, MLC protein expression decreased in model group detected by immunohistochemistry technique and Western blotting, and MLC mRNA expression decreased in model group detected by RT-PCR (P<0.01). Compared with model group, MLC protein expression increased in low-dose group, mid-dose group and high-dose group detected by immunohistochemistry technique (P<0.05, P<0.01). MLC protein expression increased in mid-dose group and high-dose group detected by Western blotting (P<0.01). MLC mRNA expression increased in high-dose group detected RT-PCR (P<0.01). Conclusion There are decreases of protein and mRNA expressions of MLC in FD-SDP rats, and Pixu Fang 1 can up-regulate these expressions.

Key words: Pixu Fang 1 (Spleen-deficiency Formula 1), functional dyspepsia, spleen deficiency pattern, gastric dynamics, myosin light chain, rats

CLC Number: 

  • R285.5