主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (9): 736-742.doi: 10.3969/j.issn.1006-2157.2017.09.006

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Anti-depressant effect and mechanism of Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula) in several mouse models of depression*

LIU Baoxiu1, WANG Pingping1, YANG Tao1, LI Lina2, LI Jianzhi2, CHANG Hongsheng1#   

  1. 1 School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 School of Basic Medical Sciences, Beijing University of Chinese Medicine, Beijing 100029, China
  • Received:2017-03-29 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    National Natural Science Foundation of China (No. 81373584)

Abstract: Objective To study the anti-depressant effect and mechanism of Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula). Methods The reserpine depression model was established by intraperitoneal injection of reserpine (2.5 mg/kg) in mice, acute stress depression model, by forced swimming test (FST) in mice, and chronic stress depression model, by long-term bandage (14 d) and noise in mice. The influences of Peiyuan Jieyu Fang on body temperature, ptosis and rate of overstepping bound in reserpine depression model, and on immobility time of FST in acute stress depression model and chronic stress depression model were observed. The content of brain 5-hydroxytryptamine (5-HT), noradrenalin (NA) and indoleamine-2, 3-dioxygenase (IDO) in acute stress depression model, and content of 5-HT and IDO in chronic stress depression model were detected by using enzyme-linked immunosorbent assay (ELISA). The expression of IDO mRNA was detected by using fluorescence quantitative polymerase chain reaction (PCR) in chronic stress depression model. Results The decrease of body temperature was antagonized in all Peiyuan Jieyu Fang groups (abbreviated as high-dose group, mid-dose group and low-dose group) in reserpine depression model (P<0.01). The ptosis and akinesia induced by reserpine were relieved in high-dose group (P<0.05), and akinesia was relieved in low-dose group (P<0.05). The immobility time of FST was shortened (P<0.05, P<0.01), and content of 5-HT and NA were significantly increased in acute stress depression model in mid-dose group and low-dose group (P<0.05, P<0.01). The immobility time of FST was shortened in varying degrees, content of 5-HT was increased, content of IDO was decreased and IDO mRNA expression was up-regulated in chronic stress depression model in high-dose group, mid-dose group and low-dose group (P<0.05). Conclusion Peiyuan Jieyu Fang has some relief effects on depression symptoms induced by reserpine, FST and chronic stress in mice. The mechanism may be related to that Peiyuan Jieyu Fang can increase the content of 5-HT and NA, inhibit IDO activity, and down-regulate IDO mRNA expression.

Key words: Peiyuan Jieyu Fang (Tonifying-original Qi Releasing-depression Formula), anti-depressant, mechanism, 5-hydroxytryptamine, noradrenalin, indoleamine-2,3-dioxygenase, mice

CLC Number: 

  • R285.5