主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (9): 743-749.doi: 10.3969/j.issn.1006-2157.2017.09.007

• CONTENTS • Previous Articles     Next Articles

Heart protective effect of Qihong Fang (Wolfberry and Hawthorn Decoction) in aging rats with cold-congealing and blood-stasis pattern*

ZHANG Rui1, FENG Xuanye1, WANG Xiang1, LIU Linlin1, DAI Ning1, FENG Lili1, SUN Yan1, ZHAO Xin2, CAI Dayong2, WANG Yueqi1#   

  1. 1 School of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China;
    2 Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China
  • Received:2017-03-27 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    Key Research Foundation from Ministry of Education (No. 108019

Abstract: Objective To discuss the influence of Qihong Fang (Wolfberry and Hawthorn Decoction) on heart in aged rats with cold-congealing and blood-stasis pattern. Methods SD rats (n=48) were divided randomly into normal control group, model group, vitamin E group (13.5 mg/kg), low-dose Qihong Fang group (45 mg/kg), mid-dose Qihong Fang group (90 mg/kg) and high-dose Qihong Fang group (180 mg/kg). The aged rat model of cold-congealing and blood-stasis pattern was established through 28-d ozone leading to aging and 14-d ice water leading to cold-congealing and blood-stasis pattern in other 5 groups except of control group. On the 29th d, the blood samples were collected from abdominal aorta for isolating serum and plasma, and heart was separated too. The vitalities of serum total superoxide dismutase (T-SOD), Cu-Zn SOD, Mn-SOD, catalase (CAT), glutathione peroxidease (GSH-PX), content of malondialdehyde (MDA), and prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT) and fibrinogen (FIB) were detected. The morphologic changes of heart cells were observed after HE staining, fiber deposition quantity was observed after Masson staining, expression of transforming growth factor β1 (TGF-β1) was detected by using immunohistochemistry technique for reviewing aging model of cold-congealing and blood-stasis pattern, and protective effect of Qihong Fang on heart was observed in rats. Results Compared with normal control group, the vitalities of T-SOD, Cu-Zn-SOD, Mn-SOD, CAT and GSH-PX decreased significantly (P<0.05), MDA content increased significantly (P<0.05), PT and APTT decreased significantly (P<0.05), and FIB content increased significantly (P<0.05) in model group. After Masson staining, morphometry observation by light microscope showed deposition of heart collagen fibers increased significantly (P<0.05), and expression of TGF-β1 increased significantly (P<0.05). Compared with control group, the vitalities of T-SOD, Cu-Zn-SOD, CAT and GSH-PX increased significantly (P<0.05), MDA content decreased significantly (P<0.05), PT and ATPP increased significantly (P<0.05), FIB content decreased significantly (P<0.05), deposition of heart collagen fibers decreased significantly (P<0.05), and expression of TGF-β1 decreased significantly (P<0.05) in mid-dose and high-dose Qihong Fang groups. Conclusion Qihong Fang has a protective effect on heart in aged rats with cold-congealing and blood-stasis pattern.

Key words: Qihong Fang (Wolfberry and Hawthorn Decoction), cold-congealing and blood-stasis, senility, heart, transforming growth factor -β1, rats

CLC Number: 

  • R285.5