主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (9): 764-771.doi: 10.3969/j.issn.1006-2157.2017.09.010

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Volatile constituents of Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil) analyzed by applying solid phase microextraction-gas chromatography-mass spectrometry*

TANG Xueyang1, QIN You1,4, CHEN Lin1,2, ZHOU Rongrong1, SHAO Xiangning4, SHI Shuyun5, TANG Chunyu6, ZHANG Shuihan1,2,3#   

  1. 1 Institute of Chinese Materia Medica, Hunan Academy of Chinese Medicine, Hunan 410013, China;
    2 Hunan Chinese Medicine Raw Material Quality Monitoring Technology Service Center, Hunan 410013, China;
    3 2011 Collaboration and Innovation Center for Digital Chinese Medicine in Hunan, Hunan 410208, China;
    4 Hunan University of Chinese Medicine, Hunan 410016, China;
    5 Central South University, Hunan 410083, China;
    6 Hunan Times Sunshine Pharmaceutical Corp., Hunan 425000, China
  • Received:2017-03-02 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    Special Project of Public Health of State Adiministration of Traditional China Medicine(Financial Society, No. [2011]-76), Hunan Academy of Chinese Medicine (No. 201601)

Abstract: Objective To analyze the volatile constituents of Linglingxiang (Lysimachia foenum-graecum, Hance, Holy Basil) by applying solid phase microextraction-gas chromatography-mass spectrometry (SPME-GC/MS). Methods The heads of solid phase microextraction (SPME) with 4 kinds of different coating, 75 μm CAR/PDMS, 65 μm DVB/PDMS, 85 μm PA and 100 μm PDMS, were used to extract the under the best extraction condition, and then these volatile constituents were detected by using gas chromatography-mass spectrometry (GC-MS). Results The key parameters of SPME for extracting volatile constituents from Linglingxiang were as follows: temperature was 85℃ and extracting time was for 50 min. A total of 10 types including 103 kinds of volatile constituents were identified by 4 different SPME extraction heads, and phenols, esters and hydrocarbons were main chemical types of volatile compounds in Linglingxiang. There were totally 6 volatile constituents detected by 4 different SPME extraction heads, and they were Sugarlactone, Dihydroactinidiolide, (+)-cedrol, phytone, phenanthrene, and 3-Amino-4, 5-dimethyl-2(5h)-furanone. Among all extraction heads, the head of 75 μm CAR/PDMS detected the largest number of volatile constituents reached to 43. Conclusion SPME-GC/MS was used for the first time form determining the volatile constituents in Linglingxiang. The method is simple, rapid and accurate, which can offer reference to further studies on the material base of Linglingxiang.

Key words: Linglingxiang (Lysimachia foenum-graecum Hance, Holy Basil), volatile constituents, solid phase microextraction, gas chromatography-mass spectrometry

CLC Number: 

  • R284.1