主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (9): 772-776.doi: 10.3969/j.issn.1006-2157.2017.09.011

• CONTENTS • Previous Articles     Next Articles

Technique optimization for extracting the total alkaloids from Baiqucai (Herba Chelidonii, Greater Celandine) by response surface analysis*

JIANG Hua1, LI Jun1#, LI Xin1, DONG Haipeng2, SHI Keyuan1, LIU Dandan1   

  1. 1 Chemical Engineering and Pharmaceutics College, Henan University of Science and Technology, Henan 471023, China;
    2 State Intellectual Property Office, Beijing 100088, China
  • Received:2017-04-24 Online:2017-09-10 Published:2017-09-10
  • Supported by:
    Natural Science Foundation of Henan Province of China (No. 162300410104)

Abstract: Objective To optimize the best technique for extracting the total alkaloids from Baiqucai (Herba Chelidonii, Greater Celandine). Methods The yield of the total alkaloids was taken as review index by using acid dye colorimetry, and Plackett-Burman design (PBD) test was applied at first for screening 3 main factors influencing the technique of extracting the total alkaloids from Baiqucai and they were ethanol volume fraction, solid-liquid ratio and extracting times. Then the best technologic conditions for extracting the total alkaloids from Baiqucai were determined by applying response surface analysis (RSA) of Box-Behnken design (BBD) test. Results The best extracting technique was as follows: ethanol volume fraction was 85%, extracting duration was 60 min, extracting temperature was 70℃, degree of grinding was 40 meshes, solid-liquid ratio was 1: 15, and extracting times were 3 times. In these conditions, the yield of the total alkaloids from Baiqucai was up to 1.92%, which was basically the same as theoretical prediction (1.95%). Conclusion The extracting technique optimized in this test is reasonable and feasible, and can get higher content of the total alkaloids

Key words: Baiqucai (Herba Chelidonii, Greater Celandine), total alkaloids, Plackett-Burman design, Box-Behnken design, extracting technique

CLC Number: 

  • R284.2