主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (10): 849-855.doi: 10.3969/j.issn.1006-2157.2017.10.011

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Effects of qi-tonifying and blood-activating therapy on mice breast cancer model with qi-deficiency blood-stasis pattern: animal model establishment and interventional study*

ZHENG Xiang1, GUO Yong2#, LI Yan3   

  1. 1 The Second Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang 310005, China;
    2 The First Affiliated Hospital of Zhejiang Chinese Medical University, Zhejiang 310006, China;
    3 Zhejiang Chinese Medical University, Zhejiang 310053, China
  • Received:2017-04-17 Online:2017-10-10 Published:2017-10-10
  • Supported by:
    Natural Science Foundation Project of Zhejiang Province(No.Y13H290038),Traditional Chinese Medicine Scientific Research Foundation Project of Zhejiang Province(No.2012ZA049)

Abstract: Objective To establish a mice model of breast cancer 4-T1 with the pattern of qi deficiency and blood stasis and study the effects of qi-tonifying and blood-activating therapy on tumor microenvironment. Methods 48 female Balb/c mice were randomly divided into 6 groups: blank control group (group A), tumor-bearing model group (group B), tumor-bearing with qi-deficiency blood-stasis pattern composite model group (group C, composite model), composite model with low-dose herbal treatment group (group D), composite model with mid-dose herbal treatment group (group E), and composite model with high-dose herbal treatment group (group F). 4-T1 monocyte suspension was injected to induce breast cancer model while reserpine injection was added to establish qi-deficiency blood-stasis composite pattern model. Herbal treatment (Si junzi Tang plus Xuefu Zhuyu Tang, i.e. four noble-ingredient decoction plus blood-activating stasis-removing decoction) of corresponding dose was offered to mice (intragastric administration) in group D, E and F from the 15th day daily for 28 days consecutively. Symptoms scores were evaluated on the 7th, 14th, 21st, 28th, 35th, and 42nd day. On the 42nd day blood flow of microcirculation in ear rim, toes and veins on the abdominal surface was detected. Matrix metalloproteinase-2 (MMP-2) and phospho-Erk1/2 (p-ERK1/2) in tumor issue were tested by using Western blot. Results Symptoms of qi deficiency and blood stasis were observed in group C, D, E and F. Symptom score of group D became lower than group C since day 28(P<0.05). The local blood flow of mice in group A was higher than that of group C fom day 21 (P<0.01). Blood flow in group C was lower than that in group B (P<0.05). Microcirculation all improved in group D, E and F compared with group B (P<0.01 or P<0.05). The expression of MMP-2 in mice in group C was higher than that in group B(P<0.05); lower in group D, E and F than that in group C(P<0.05). The expression of p-ERK1/2 in group C was slightly higher than that in group B(P>0.05); lower in group D and E than that in group C (P<0.01 or P<0.05). Conclusions The mice composite model of breast cancer with qi deficiency and blood stasis established in this study proves to be reliable and consistent with TCM pattern characteristic. MMP-2 and p-ERK1/2 were upregulated in mice tumor with the pattern of qi deficiency and blood stasis. Qi-tonifying and blood-activating herbal intervention could not only relive qi deficiency and blood stasis, but also downregulate the expression of MMP-2 and p-ERK1/2, and thus inhibit the growth of tumor.

Key words: breast cancer, qi deficiency and blood stasis, qi-tonifying and blood-activating therapy, MMP-2, p-ERK1/2, mice

CLC Number: 

  • R285.5