主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (11): 923-927.doi: 10.3969/j.issn.1006-2157.2017.11.009

• Orignal Article • Previous Articles     Next Articles

Influence of Yiqi Huoxue Tongluo Fang on JNK signal pathway and inflammatory factors in mice with diabetic peripheral neuropathy*

YAO Yongchuan1, SHEN Guoming2#, JIANG Aijuan2, WANG Liang2, YE Shu1, WANG Liu1   

  1. 1 Clinical School of Integrated Traditional Chinese and Western Medicine, Anhui University of Traditional Chinese Medicine, Anhui 230038, China;
    2 Graduate School, Anhui University of Traditional Chinese Medicine, Anhui 230038, China
  • Received:2017-05-22 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation (No. 81273646), Natural Science Foundation of Anhui Province(No.1608085MH232)

Abstract: ObjectiveTo study the influence of Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction) on signal pathway of c-jun N-terminal kinase (JNK) and inflammatory factors in mice with diabetic peripheral neuropathy (db/db mice), and discuss its mechanism in protecting peripheral nerve. Methods The db/db mice (n=40) were randomly divided into 5 groups: model group, lipoic acid group, high-dose Yiqi Huoxue Tongluo Fang group (6.4 g/kg, high-dose group), mid-dose Yiqi Huoxue Tongluo Fang group (3.2 g/kg, mid-dose group) and low-dose Yiqi Huoxue Tongluo Fang group (1.6 g/kg, low-dose group), and other db/m mice (n=8) were chosen into normal group. All groups were intragastrically given corresponding drugs once a day for 8 weeks. After the last administration of drugs, the plantar thermal response time and motor nerve conduction velocity (MNCV) were detected. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by using enzyme-linked immunosorbent assay (ELISA). The protein expressions of JNK and phosphorylase JNK (p-JNK) in dorsal root ganglion (DRG) were detected by using Western blot method. Results Compared with normal group, MNCV was slower, plantar thermal response time was longer, levels of IL-1β and TNF-α increased, and protein expressions of JNK and p-JNK are stronger in model group (P<0.01 or P<0.05). Compared with model group, Yiqi Huoxue Tongluo Fang promoted MNCV, shortened plantar thermal response time, decreased levels of IL-1β and TNF-α and down-regulated protein expressions of JNK and p-JNK to some extent (P<0.01 or P<0.05). ConclusionYiqi Huoxue Tongluo Fang can inhibit the abnormal activation of JNK signal pathway and relieve inflammatory reaction to protect peripheral nerve in db/db mice.

Key words: Yiqi Huoxue Tongluo Fang (Qi-Replenishing Blood-Activating Collateral-freeing Decoction), db/db mice, diabetic peripheral neuropathy, JNK signal pathway, inflammatory factors

CLC Number: 

  • R285.5