主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (11): 928-932.doi: 10.3969/j.issn.1006-2157.2017.11.010

• Orignal Article • Previous Articles     Next Articles

Microenvironment of peripheral nerve regeneration supported by velvet antler polypeptides-PLGA compound membrane*

WANG Keli1, ZHANG Jingling2, LU Laijin3   

  1. 1 Department of Orthopedics, General Hospital of Chinese PLA Rockets Force, Beijing 100088, China;
    2 Hospital of University of Science & Technology,Beijing 100086, China;
    3 First Clinical College of Jilin University, Jilin 130021, China
  • Received:2017-05-11 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation of China (No. 30470833)

Abstract: Objective To investigate the influence of velvet antler polypeptides (VAP)-PLGA compound membrane formed from VAP and copolymer (PLGA) of polylactic acid (PLA) and polyglycolic acid (PGA) on regeneration of sciatic nerve in rats. Methods Healthy male Wistar rats (n=36) were randomly divided into 4 groups (each n=9). The sciatic nerve of rat was revealed by operation, and was not treated in sham-operation group after isolating the nerve and sewed up directly broken ends in model group after cutting the nerve. The sciatic nerve of rat was sewed up and wrapped up with PLGA compound membrane in control group and the nerve was sewed up and wrapped up with VAP-PLGA compound membrane in treatment group. The samples were collected respectively on the 2nd, 4thand 6th weeks after the operation and given histological and immunohistochemical observation and detection. Results The histological observation showed that nervous axon was normal in sham-operation group at 3 time points. The quantity of medullated fibers was significantly more, and axon regeneration rate and maturity of regenerated axon were significantly higher in treatment group than those in model group and control group (P<0.05) at 3 time points. The immunohistochemical detection showed that the antigen staining and antigen expressions of transforming growth factor β (TGF-β) and insulin-like growth factor (IGF) in regenerated nerve fiber axon and myelin sheath were significantly higher in treatment group than those in sham-operation group, model group and control group (P<0.05). Conclusion VAP-PLGA compound membrane can provide a microenvironment and neuroactive factors required by neural restoration, and promote the regeneration of peripheral nerve, which is an ideal biological material for neural restoration.

Key words: velvet antler polypeptides, PLGA , peripheral nerve, nerve regeneration, rats

CLC Number: 

  • R285.5