主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (11): 933-939.doi: 10.3969/j.issn.1006-2157.2017.11.011

• Orignal Article • Previous Articles     Next Articles

Regulation on autophagy with tanshinone ⅡA for anti-oxidative stress damage of endothelial cells through PI3K/Akt/mTOR pathway*

CAO Huimin1, SONG Nan1, ZHANG Ni1, YANG Guanlin1, CHEN Wenna1, ZHANG Zhe2, JIA Lianqun1#   

  1. 1 Key Laboratory of Ministry of Education for TCM Viscera-State Theory and Applications, Translational Medicine Research Center of traditional Chinese medicine in Liaoning Province, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Liaoning 110032, China
  • Received:2017-05-22 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation for Young Scientists of China (No. 81300229), Natural Science Foundation of Liaoning Province (No. 2015020394), Support Programme for Talents in Colleges and Universities of Liaoning Province (No. LR2015041)

Abstract: Objective To investigate the protective and mechanism of tanshinone ⅡA (TSⅡA) in oxidative stress damage of endothelial cells induced by oxidized low-density lipoprotein (ox-LDL) based on PI3K/Akt/mTOR autophagy pathway. Methods EA. hy926 cells were cultured in vitro, and then randomly divided into normal group, model group, ox-LDL+TSⅡA group, ox-LDL+TSⅡA+LY294002 group, TSⅡA group and LY294002 group. The content of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were detected by using chromatoptometry, autophagy status was observed by using FITC-LC3 fluorescence microscope and Naolive 3D Cell Explorer Real-time Microscopic Imaging System, and meanwhile, expressions of autophagy-related proteins and PI3K/Akt/mTOR pathway proteins were detected by using Western Blotting. Results Compared with normal group, MDA content increased, SOD activity decreased and content of LC3-I/LC3-II protein increased in model group (P<0.01), and after TSⅡA intervention, MDA content decreased, SOD activity increased and content of LC3-I/LC3-II protein increased (P<0.01). Compared with normal group, protein expressions of PI3K, p-Akt and p-mTOR decreased significantly in model group (P<0.05 or P<0.01). Compared with model group, protein expressions of PI3K, p-Akt and p-mTOR decreased significantly after TSⅡA intervention (P<0.05 or P<0.01). Compared with ox-LDL+TSⅡA group, content of LC3-I/LC3-II protein decreased and autophagy level decreased (P<0.01) after applying LY294002, an inhibitor of PI3K. Conclusion Tanshinone ⅡA may improve autophagy through regulating PI3K/Akt/mTOR pathway to relieve the oxidative stress damage of EA. Hy926 cells and prevent atherosclerosis.

Key words: tanshinone ⅡA, oxidized low-density lipoprotein, atherosclerosis, oxidative stress, autophagy

CLC Number: 

  • R285.5