主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2017, Vol. 40 ›› Issue (11): 946-953.doi: 10.3969/j.issn.1006-2157.2017.11.013

• Orignal Article • Previous Articles     Next Articles

Quality analysis on fresh Danshen (Radix Salviae Miltiorrhizae, Red Sage Root) from different producing areas based on technology of high-resolution liquid chromatography-mass spectrometry*

SHAO Yuanyang1, XU Guojie1,2, JIN Xiaoyan1, LIU Siqi3, ZHANG Chi1, WANG Juan1, MA Shuangshuang1, ZHANG Caijuan1, QIU Minyi1, LIU Chunsheng1, WANG Xueyong1#   

  1. 1 School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;
    2 School of Life Sciences, Beijing University of Chinese Medicine, Beijing 100029, China;
    3 Health Service Center, Guajiasi Community, Hexi District, Tianjin 300210, China
  • Received:2017-06-06 Online:2017-11-10 Published:2017-11-10
  • Supported by:
    National Natural Science Foundation of China (No. 81274011), Central Major Adding and Removing Project (No. 2060302), Natural Science Foundation of Beijing (No. 7152094)

Abstract: Objective To analyze the content of active components in fresh Danshen (Radix Salviae Miltiorrhizae, Red Sage Root) from different producing areas, and review reasonably the medicinal quality of fresh Danshen. Methods The content of 5 salvianolic acids and 12 tanshinones in fresh Danshen, from 7 different producing areas, were determined by applying LTQ-Orbitrap XL high-resolution mass spectrometer. On this basis, all content were analyzed by using principal component analysis (PCA) and partial least square (PLS) analysis. Results The content of active components in fresh Danshen from the same producing area were basically the same, and difference was significant in content of active components among fresh Danshen from different producing areas. The content of salvianolic acids was the highest in fresh Danshen from Hubei, and content of tanshinone I and tanshinone IIA were the highest in fresh Danshen from Sichuan and Henan. The analysis results of PCA and PLS showed that the distribution of most active components had a distinct regional specificity. Conclusion The difference is significant in quality of fresh Danshen from different producing areas, and they have a distinct regional specificity. Using fresh Danshen as quality evaluation materials, that is a useful supplement to review quality of raw medicinal materials based on dry herbs. It explored a new and more comprehensive approach for reviewing other Chinese medicinal materials.

Key words: Danshen (Radix Salviae Miltiorrhizae, Red Sage Root), content determination, principal component analysis, partial least square analysis, variation

CLC Number: 

  • R284.1