主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (1): 53-59.doi: 10.3969/j.issn.1006-2157.2018.01.009

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Melanosis of colon induced by different laxatives in guinea pigs: an experimental study*

Yu Qiang, Liu Wei, Jiang Jing, Liu Renhai#   

  1. DongFang Hospital, Beijing Unirorsity of Chinese Medicine, Beijing 100078,China;
  • Received:2017-07-12 Online:2018-01-30 Published:2018-01-30
  • Supported by:
    National Natural Science Foundation of China (No. 81273757)

Abstract: Objective To discuss the pathogenesis of melanosis of colon (MC). Methods SPF guinea pigs were randomly divided into 4 groups: normal group (given water,2 mL n=8), group of Dahuang (Radix et Rhizoma Rhei, Rhubarb; Dahuang group, 1.16 g/kg n=16), group of Fanxieye (Folium Sennae, Senna Leaf, Fanxieye group, 0.46 g/kg n=16) and Phenolphthalein Tablets group (15.43 mg/kg n=16), and all groups were intragastrically given corresponding medicinal. The half numbor of guinea pigs was executed on the 4th week and other half were executed on the 8th week. The status of colonic pigmentation of guinea pigs in each group was observed by direct visual observation and melanin staining. The colonic histopathology of guinea pigs in each group was observed by the method of HE staining. The apoptosis of colon tissue was detected by using terminal-deoxynucleoitidyl transferase mediated nick end labeling (TUNEL), and level of tumor necrosis factor-α (TNF-α) was detected by using enzyme-linked immunosorbent assay (ELISA) in all groups. Results There was MC occurred in varying degrees in Dahuang group, Fanxieye group and Phenolphthalein Tablets group after 4 weeks and 8 weeks (P<0.01). MC aggravated along with time increased, however, there was statistical difference in MC severity only between Fanxieye group and normal group after 4 weeks and 8 weeks (P<0.05). The value of optical density (OD) was significantly higher in Dahuang group, Fanxieye group and Phenolphthalein Tablets group than that in normal group after 4 weeks and 8 weeks (P<0.01), and along with time increased, OD value also increased but had no statistical difference (P>0.05). The level of TNF-α was higher in Dahuang group than that in normal group after 4 weeks and 8 weeks (P<0.05), and increased significantly along with time increased (P<0.01). The level of TNF-α had no statistical difference between Fanxieye group and normal group after 4 weeks (P>0.05), was higher in Fanxieye group than that in normal group after 8 weeks (P<0.05), and increased significantly along with time increased (P<0.01). The level of TNF-α had no statistical difference between Phenolphthalein Tablets group and normal group after 4 weeks and 8 weeks (P>0.05), and along with time increased, level of TNF-α also increased but had no statistical difference (P>0.05). Conclusion Dahuang, Fanxieye and Phenolphthalein Tablets all can induce MC in guinea pigs, and the pathogenesis may be related to apoptosis of colonic epithelial cells. But the relationship between MC and level of TNF-α needs further research.

Key words: melanosis of colon, apoptosis, tumor necrosis factor-α, Dahuang (Radix et Rhizoma Rhei, Rhubarb), Fanxieye (Folium Sennae, Senna Leaf), Phenolphthalein Tablets, guinea pigs

CLC Number: 

  • R285.5