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ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (1): 60-64.doi: 10.3969/j.issn.1006-2157.2018.01.010

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Influence of medicated serum of Liuwei Dihuang Tang on PHD2/HIF-1α signal pathway in HK-2 cells*

Tang Qun1, Wu Hua2#, Liu Chunyan1, Luo Lin1, Yi Chun1, Xu Wenfeng3, Zhuang Gaojian1   

  1. 1 Department of Pathology, Hunan University of Chinese Medicine, Hunan 410208, China;
    2 Furong Judicial Identification Center, Hunan Second People’s Hospital, Hunan 410007, China;
    3 Department of Nephrology, First Affiliated Hospital of Hunan University of Chinese Medicine, Hunan 410007, China;
  • Received:2017-07-28 Online:2018-01-30 Published:2018-01-30
  • Supported by:
    National Natural Science Foundation of China (No. 81503442、No. 81603470), Education Department of Hunan Province (No. 17B197, No. 16B196)

Abstract: Objective To investigate the effect of PHD2/HIF-1 signaling pathway on the renal interstitial fibrosis, and the intervention effect of medicated serum of Liuwei Dihuang Tang on HK-2 cells induced by hypoxia. Methods A total of 40 SPF male SD rats were divided into blank group and medicated group (each n=20). The blank group was orally given normal saline 〔10 mL/(kg·d)〕 and medicated group was orally given Liuwei Dihuang Tang for 1 week continuously, and then blood samples were collected from rat abdominal aorta for separating serum after centrifugation. HK-2 cells were cultured in vitro. HK-2 cells were divided into blank control group (10% of fetal bovine serum), normal group (10% of normal serum), CoCl2 group (150 μmol/L of CoCl2+10% of normal serum) and medicated serum group (150 μmol/L of CoCl2+10% of medicated serum of Liuwei Dihuang Tang). HK-2 cells were cultivated in vitro and treated in all groups for 24 h, and then the influence of medicated serum of Liuwei Dihuang Tang on protein levels and mRNA expressions of PHD2, HIF-1α and connective tissue growth factor (CTGF) in HK-2 cells were detected, respectively, by using Western blotting method and reverse transcription polymerase chain reaction (RT-PCR). Results Compared with normal group, the protein level and mRNA expression of PHD2 in HK-2 cells all decreased (P<0.05), while protein levels of HIF-1α and CTGF and mRNA expressions of HIF-1α and CTGF increased (P<0.05) in CoCl2 group. Compared with CoCl2 group, the protein level and mRNA expression of PHD2 increased (P<0.05), and those of HIF-1α and CTGF decreased (P<0.05) in medicated serum group. Conclusion PHD2/HIF-1α signal pathway takes part in RIF, and Liuwei Dihuang Tang can inhibit RIF through up-regulating PHD2 expression, improving HIF-1α degradation and down-regulating CTGF expression.

Key words: HK-2 cells, renal interstitial fibrosis, proline hydroxylase 2, hypoxia-inducible factor 1α, connective tissue growth factor, medicated serum of Liuwei Dihuang Tang (Six-Ingredient Rehmannia Decoction) , rats

CLC Number: 

  • R285.5