主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (1): 65-70.doi: 10.3969/j.issn.1006-2157.2018.01.011

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Influence of metformin combined with Gexia Zhuyu Tang on inflammatory factors and sex hormone level in rats with polycystic ovarian syndrome*

Jiang Xiaolin1, Zhang Bing2, Hou Haiyan3, Teng Fei4, Ma Xiande4, Lyu Meijun1, Wang Ying1, Chu Chunli5, Dong Xiaoqian5, Zhang Lide1#   

  1. 1 Key Laboratory of Ministry of Education for TCM Visceral manifestation Theory and Applications, Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    2 Hospital of Chinese Medicine of Liaoning Province, Liaoning 110032, China;
    3 Logistics College of Chinese People’s Armed Police, Tianjin 300162, China;
    4 Liaoning University of Traditional Chinese Medicine, Liaoning 110847, China;
    5 Liaoning Institute of Traditional Chinese Medicine, Liaoning 110000, China;
  • Received:2017-02-19 Online:2018-01-30 Published:2018-01-30
  • Supported by:
    National Natural Science Foundation of China (No. 81402691), Innovation Team of Liaoning Provincial Education Department (No. LT2014021), Key Laboratory of Ministry of Education for TCM Visceral manifestation Theory and Applications (No. 11020163150009)

Abstract: Objective To study the influence of metformin combined with Gexia Zhuyu Tang (Decoction for Dissipating Blood Stasis under Diaphragm) on serum inflammatory factors, sex hormone level and glycolipid metabolism in rat model of polycystic ovarian syndrome (PCOS) induced by dehydroepiandrosterone. Methods PCOS rats (n=69) were randomly divided into control group (n=34) and intervention group (n=35). The control group was intragastrically given metformin (270 mg/kg) and intervention group, metformin (270 mg/kg) and Gexia Zhuyu Tang (34.5 mg/kg) for continuous 28 d. The changes of serum inflammatory factors [interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP)], blood fat [triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C) and total cholesterol (TC)], blood sugar [fasting plasma glucose (FPG) and 2-h postprandial blood glucose (2-hPBG)], and sex hormone level [follicle-stimulating hormone (FSH), luteinizing hormone (LH), estrogen (E2) and testosterone (T) were detected by using enzyme-linked immunosorbent assay (ELISA). The protein expressions of IL-6 and TNF-α were detected by using immunohistochemistry technique. Results After intervention, the levels of IL-6, TNF-α, CRP, LH, LH/FSH, E2, T, TG, LDL-C, TC, FPG and 2 hPG all decreased in 2 groups (P<0.05), and they were all lower in intervention group than those in control group (P<0.05). The levels of HDL-C and FSH increased in 2 groups after intervention (P<0.05), and they were higher in intervention group than those in control group (P<0.05). The protein expressions of IL-6 and TNF-α were lower in intervention group than those in control group (P<0.05). Conclusion Metformin combined with Gexia Zhuyu Tang can reduce the levels of peripheral serum inflammatory factors and meliorate levels of blood fat, blood sugar and hormones in PCOS rats, and the effect of which is better than that ot sinle metformin.

Key words: metformin, Gexia Zhuyu Tang (Decoction for Dissipating Blood Stasis under Diaphragm), polycystic ovarian syndrome, inflammatory factors, hormone level, glycolipid metabolism, rats

CLC Number: 

  • R285.5