主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (1): 76-82.doi: 10.3969/j.issn.1006-2157.2018.01.013

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Expression of miR-122-related factors of liver fibrosis regulated by Fuzheng Huayu Tablet*

Zhang Wei1, Shao Mingliang1, Zhang Haicong1, Wu Zongyao1, Miao Tongguo1, Zheng Huanwei1,Xie Xingtong2, Wang Rui2#   

  1. 1 Research Center, Fifth Hospital of Shijiazhuang City, Hebei 050021, China;
    2 Hebei University of Traditional Chinese Medicine, Hebei 050200, China;
  • Received:2017-07-11 Online:2018-01-30 Published:2018-01-30
  • Supported by:
    National Science and Technology major projects (No.2014ZX10005001-009),Science and Technology Projects of Hebei Province (No.17277730D)

Abstract: Objective To investigate softening-hardness dissipating-binds effects of Fuzheng Huayu (reinforcing-healthy qi resolving-stasis) Tablet combined with anti-virus therapy on liver fibrosis, and relationship between these effects and regulation of pathway of miR-122-interleukin-10-reactive oxygen species (miR-122/IL-10/ROS). Methods The patients with chronic hepatitis and liver fibrosis (n=85) were chosen from Aug. 2015 to Aug. 2016, and then divided randomly into test group (n=45) and control group (n=40). The control group was treated with entecavir (0.5 mg/d) and monoammonium glycyrrhizinate (0.1 g/d), and test group, additionally with Fuzheng Huayu Tablet (4.5 g/d) for 48 weeks. After treatment, ISHAK fibrosis score was reviewed through routine pathology, and activities of serum superoxide dismutase (SOD) and ROS were detected by using ELISA. The expressions of miR-122 mRNA and IL-10 mRNA were detected by using RT-PCR, and content of serum inflammatory factors of liver fibrosis, including procollagen III N-terminal peptide (PCIIINP), type IV collagen (IV-C), hyaluronidase (HA) and laminin (LN), were detected by using immunoturbidimetry. Results ISHAK fibrosis score had no significant difference between 2 groups before treatment, and decreased in 2 groups after treatment, which was superior in test group to that in control group (P<0.05). The content of serum inflammatory factors of liver fibrosis all decreased in 2 groups after treatment, and the decreases of PCIIINP and HA were more significant in test group compared with control group (P<0.05). The activities of ROS and SOD had no difference in 2 groups before treatment, and ROS decreased in 2 groups after treatment. The inhibitory effect on ROS was better in test group than that in control group after treatment (P<0.05), and SOD increase was effectively relieved in test group after treatment (P<0.05). The expressions of miR-122 mRNA and IL-10 mRNA all decreased in 2 groups after treatment, which was more significant in test group compared with control group (P<0.05). Conclusion Fuzheng Huayu Tablet reduces directly the levels of collagen and HA, degrades abnormal deposition of extracellular matrix, and regulates dynamic balance of collagen, and it antagonizes effectively the genetic expressions of IL-10 and miR-122, relieves inflammatory disorders and controls progress of histology.

Key words: liver fibrosis, miR-122, transforming growth factor, interleukin-10, reinforcing healthy qi and resolving stasis

CLC Number: 

  • R259.752