主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (2): 124-130.doi: 10.3969/j.issn.1006-2157.2018.02.006

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Effects of baicalein on AC-cAMP-PKA signaling pathway in prefrontal cortex of rats with attentional deficit hyperactivity disorder*

Wang Qing1, Fan Jinping2, Liao Yingzhao3, Chen Jing3, Liu Hua3, Ni Xinqiang3#
  

  1. 1 Traditional Chinese medicine college, Hainan Medical University, Haikou 571199, China;
    2 Traditional Chinese medicine hospital of Haikou, Haikou 570216, China;
    3 Traditional Chinese medicine hospital of Shenzhen, Shenzhen 518000, China;
  • Received:2017-08-05 Online:2018-02-28 Published:2018-02-28
  • Supported by:
    National Natural Science Foundation of China (No. 81503616), Science and Technology Planning Project of Guangdong Province of China (No. 2014A020221003), Project of Guangdong Province Administration of Traditional Chinese Medicine (No. 20161218), Science and Technology Project of Shenzhen (No. JCYJ20160428105935612), Project of Health and Family Planning Commission of Shenzhen Municipality (No. 201505019),Research Cultivation Fund of Hainan Medical University Supported Project(No.HY2018-34)

Abstract: Objective To study the effects of baicalein on spontaneous activity, impulsivity, learning-memorial ability, and the adenylate cyclase (AC)-cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling pathway in prefrontal cortex (PFC) of rats with attention deficit hyperactivity disorder (ADHD). Methods Fifty 4-week-old SHR rats were randomly divided into the model group, methylphenidate (MPH) group (2 mg/kg), and low-dose, mid-dose and high-dose (100, 200, 400 mg/kg) baicalein groups, and WKY rats of the same age were selected as the normal control group (n=10 in each group). Rats were orally gavaged for 4 weeks in the dark-phase. Open field test (OFT), elevated plus maze (EPM) and novel object recognition test (NORT) were conducted to evaluate the spontaneous activity, impulsivity and learning-memorial ability of rats individually at the end of treatment. AC, cAMP and PKA quantity were determined with enzyme-linked immunosorbent (ELISA) method. Results Movement distance in OFT was reduced in high-dose baicalein group and mid-dose baicalein group (P<0.05) afterwards. Vertical movements also decreased in all baicalein groups. Percentage of the times entering the open arms in the EPM was significantly reduced in the mid-dose baicalein group (P<0.05); staying duration in the total period in the EPM was also reduced in all baicalein groups(P<0.05). The preference index in the testing phase of NORT increased in the mid-dose baicalein group (P<0.05). Levels of AC and cAMP in the PFC increased in all baicalein groups (P<0.05), while tPKA increased in high-dose and mid-dose baicalein group (P<0.05). Conclusion Baicalein could increase the levels of AC, cAMP and PKA in PFC of ADHD rats, which may shed some light to the mechanism how baicalein could relieve the spontaneous activity and impulsivity, improve learning-memorial ability of ADHD rats.

Key words: attentional deficit hyperactivity disorder, baicalein, AC, cAMP, PKA, rats

CLC Number: 

  • R285.5