主 办:北 京 中 医 药 大 学
ISSN 1006-2157 CN 11-3574/R

JOURNAL OF BEIJIGN UNIVERSITY OF TRADITIONAL CHINE ›› 2018, Vol. 41 ›› Issue (2): 154-159.doi: 10.3969/j.issn.1006-2157.2018.02.010

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Effects of electroacupuncture on the expression of caspase-12 in hippocampus and serum of rats with chronic unpredictable mild stress*

Tian Zhaoyang, Tu Ya, Jiang Huili, Zhang Xuhui, Lu Jun, Yu Qiuyun, Liang Xingchen, Shao Shujun, Xiang Dulian, Wu Jihong#
  

  1. School of Acupuncture-Moxibustion and Tuina, Beijing University of Chinese Medicine, Beijing 100029, China;
  • Received:2017-08-08 Online:2018-02-28 Published:2018-02-28
  • Supported by:
    National Natural Science Foundation of China(No. 81173334)

Abstract: Objective To observe the effects of electroacupuncture (EA) on the expression of central and peripheral cysteine aspartic acid protease-12 via JNK signal transduction pathway in rats with chronic stress depression and to explore anti-depression mechanism of electro-acupuncture. Methods SD rats were randomly divided into blank group, stress model group, acupuncture group, fluoxetine group, model+DMSO (dimethyl sulfoxide, soluble organic solvent) group, model+JNK pathway blocker SP600125 (hereinafter abbreviated as SP) group, acupuncture+SP group, and fluoxetine+ SP group (n=9 in each group). The combination of chronic stress and solitary raising was used to establish chronic unpredictable mild stress model in seven groups except the control group. The investigated intervention was EA at “Neiguan(PC6)” and “Sanyinjiao(SP6)” points, 20 min for each session, once a day, for 21 consecutive days. The positive control group was treated with fluoxetine 5 mg/kg by oral gavage for 21 days. Open field test was used to observe the rats’ behaviors. The expression of caspase-12 protein in hippocampus and serum was measured by using ELISA. Results Before the experiment, there was no statistic difference in rat behaviors of all groups (P>0.05). At Day 21 of the experiment, the horizontal crossing lattice and frequency of vertical movements markedly decreased compared to the blank group (P<0.01). Compared with the model group, EA increased the horizontal crossing lattice (P<0.05). Compared with the blank group, the expression of caspase-12 protein in the model group increased significantly (P<0.01). Compared with the model group, the expression of caspase-12 protein in the model+DMSO group, fluoxetine group, fluoxetine+SP group, acupuncture group and SP group all decreased (P<0.05). Compared with the blank group, the expression of caspase-12 protein in the model group decreased. Compared with the model group, the expression of caspase-12 protein in the model+DMSO group, model+SP group, fluoxetine group and acupuncture+SP group (P> 0.05) decreased, while the expression of caspase-12 in fluoxetine+SP group and acupuncture group was higher than that in the control group, but all the differences were not statistically significant (P>0.05). Conclusion Acupuncture treatment may reduce the expression of caspase-12 protein in the hippocampus of chronic stress rats by effectively inhibiting the JNK signal transduction pathway activity and reducing the apoptosis of hippocampal neuron, which contributes to its anti-depression mechanism.

Key words: depression, chronic stress, acupuncture, JNK signaling pathway, caspase-12, rats

CLC Number: 

  • R285.5